| Literature DB >> 24291453 |
H Zhang1, J Ke1, T Shao1, J Li1, Y Duan2, Y He1, C Zhang1, G Chen3, G Sun4, X Sun5.
Abstract
Significant cytotoxic effects of procynadins from chestnut (Castanea mollissima Bl.) shell (CSPC) on human hepatoma G2 (HepG2) cells were found in vitro. CSPC could inbibit HepG2 proliferation in a dose-dependent manner (100-400 μg/mL), arrest cell cycle in the G0/G1 phase, induce apoptosis and trigger necrosis of HepG2. Proapoptotic effect of CSPC was evidenced by nuclear condensation, internucleosomal DNA fragmentation. Treatment of HepG2 cells with CSPC caused a loss of mitochondrial membrane potential and stimulated reactive oxidative species (ROS) generation. These results suggested CSPC could trigger apoptosis and necrotic cell death in HepG2 cell, which might be associated with ROS generation through the mitochondria-dependent signaling way.Entities:
Keywords: (hydroxymethyl) aminomethane; 2,7-dichlorofluorescein; 2′,7′-dichlorofluorescein diacetate; 4′, 6-diamidine-2′-phenylindole dihydrochloride; AO; Apoptosis; CCl(4); CSPC; Chestnut shell; DAPI; DCF; DCFH-DA; DL-dithiothreitol; DMEM; DMSO; DTT; Dulbecco’s modified eagles medium; EB; EDTA; ESI-MS; FCS; HCC; HFD; HPLC; HepG2; HepG2 cells; LC-ESI-MS; MDA; MTT; NP40; PBS; PI; Procyanidins; RNase; ROS; RP-HPLC; Tris; acridine orange; dimethyl sulphoxide; electrospray ionization mass spectrometry; ethidium bromide; ethylenediamine tetraacetic acid; fetal calf serum; hepatocellular carcinoma; hepatoma G2; high performance liquid chromatography; high-fat diet; liquid chromatography electrospray ionization mass spectrometry; malondialdehyde; methythiazoletetrazolium; nonylphenol polyoxyethylene ether; phosphate buffered solution; procynadins from chestnut shell; propidium iodide; reactive oxidative species; reversed-phase high performance liquid chromatography; ribonuclease; t-BHP; tert-butyl hydroperoxide; tetrachloride
Mesh：
Substances：
Year: 2013 PMID： 24291453 DOI： 10.1016/j.fct.2013.11.026
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023