J F Tahmassebi1, N Chrysafi2, M S Duggal2. 1. Department of Paediatric Dentistry, Leeds Dental Institute, Leeds, UK. Electronic address: j.tahmassebi@leeds.ac.uk. 2. Department of Paediatric Dentistry, Leeds Dental Institute, Leeds, UK.
Abstract
OBJECTIVE: This study investigated the effect of ozone on the progression or regression of artificial caries-like lesions on enamel following pH cycling conditions in vitro. METHODS: A randomized, single blind, four legs design was used. 20 full thickness enamel slabs were allocated to each of the four groups which were: Fluoride free toothpaste (control); ozone alone; Reductant/Patient Kit alone and a combination of both ozone/Reductant/Patient Kit. Artificial lesions were created and subjected to the pH cycling regime for a 14 days period. Assessments were carried out before and after the pH cycling on the slabs using the microhardness testing and Quantitative Light-induced Fluorescence (QLF). RESULTS: Statistical significant difference were found in the percentage change of enamel microhardness before and after pH cycling between ozone/Reductant/Patient Kit group and all the other three groups of the study, as well as between Reductant/Patient Kit group and control. There was a statistical significant difference in the change of size and severity of the lesion (ΔQ) between all the three regimes tested and the control with a trend favouring ozone/Reductant/Patient Kit group. CONCLUSIONS: In our model, it appeared that ozone treatment alone is not effective in protecting the enamel against demineralisation or promoting remineralisation, unless combined with the Reductant/Patient Kit, which contain high levels of fluoride.
OBJECTIVE: This study investigated the effect of ozone on the progression or regression of artificial caries-like lesions on enamel following pH cycling conditions in vitro. METHODS: A randomized, single blind, four legs design was used. 20 full thickness enamel slabs were allocated to each of the four groups which were: Fluoride free toothpaste (control); ozone alone; Reductant/PatientKit alone and a combination of both ozone/Reductant/PatientKit. Artificial lesions were created and subjected to the pH cycling regime for a 14 days period. Assessments were carried out before and after the pH cycling on the slabs using the microhardness testing and Quantitative Light-induced Fluorescence (QLF). RESULTS: Statistical significant difference were found in the percentage change of enamel microhardness before and after pH cycling between ozone/Reductant/PatientKit group and all the other three groups of the study, as well as between Reductant/PatientKit group and control. There was a statistical significant difference in the change of size and severity of the lesion (ΔQ) between all the three regimes tested and the control with a trend favouring ozone/Reductant/PatientKit group. CONCLUSIONS: In our model, it appeared that ozone treatment alone is not effective in protecting the enamel against demineralisation or promoting remineralisation, unless combined with the Reductant/PatientKit, which contain high levels of fluoride.
Authors: Thalyta Dos Reis Furlani Zouain Ferreira Neves Dias; Francisco Ubiratan Ferreira de Campos; Cecilia Pedroso Turssi; Flávia Lucisano Botelho do Amaral; Fabiana Mantovani Gomes França; Roberta Tarkany Basting Journal: Med Gas Res Date: 2022 Jul-Sep
Authors: Mahmoud K Al-Omiri; Nasser M Alqahtani; Nasser M Alahmari; Raed Abul Hassan; Abdullah A Al Nazeh; Edward Lynch Journal: Sci Rep Date: 2021-05-27 Impact factor: 4.379