PURPOSE: The use of resting-state functional MRI (rsfMRI) in preclinical research is expanding progressively, with the majority of resting-state imaging performed in anesthetized animals. Since anesthesia may change the physiology and, in particular, the neuronal activity of an animal considerably, it may also affect rsfMRI findings. Therefore, this study compared rsfMRI data from awake mice with rsfMRI results obtained from mice anesthetized with α-chloralose (120 mg/kg), urethane (2.5 g/kg), or isoflurane (1%). METHODS: Functional connectivity (FC) was estimated using both independent component analysis (40 components) and ROI-based analysis to zoom in on the effect of different anesthetics on inter-hemispheric FC. RESULTS: The data revealed an important diminishment of cortical interhemispheric FC in both the α-chloralose and urethane groups in comparison with the isoflurane and awake groups. CONCLUSION: When performing FC analysis in anesthetized mice, the impact of anesthetics must be taken into account. The required doses for stable anesthesia during MRI significantly decrease interhemispheric FC.
PURPOSE: The use of resting-state functional MRI (rsfMRI) in preclinical research is expanding progressively, with the majority of resting-state imaging performed in anesthetized animals. Since anesthesia may change the physiology and, in particular, the neuronal activity of an animal considerably, it may also affect rsfMRI findings. Therefore, this study compared rsfMRI data from awake mice with rsfMRI results obtained from mice anesthetized with α-chloralose (120 mg/kg), urethane (2.5 g/kg), or isoflurane (1%). METHODS: Functional connectivity (FC) was estimated using both independent component analysis (40 components) and ROI-based analysis to zoom in on the effect of different anesthetics on inter-hemispheric FC. RESULTS: The data revealed an important diminishment of cortical interhemispheric FC in both the α-chloralose and urethane groups in comparison with the isoflurane and awake groups. CONCLUSION: When performing FC analysis in anesthetized mice, the impact of anesthetics must be taken into account. The required doses for stable anesthesia during MRI significantly decrease interhemispheric FC.
Authors: Joanes Grandjean; Carola Canella; Cynthia Anckaerts; Gülebru Ayrancı; Salma Bougacha; Thomas Bienert; David Buehlmann; Ludovico Coletta; Daniel Gallino; Natalia Gass; Clément M Garin; Nachiket Abhay Nadkarni; Neele S Hübner; Meltem Karatas; Yuji Komaki; Silke Kreitz; Francesca Mandino; Anna E Mechling; Chika Sato; Katja Sauer; Disha Shah; Sandra Strobelt; Norio Takata; Isabel Wank; Tong Wu; Noriaki Yahata; Ling Yun Yeow; Yohan Yee; Ichio Aoki; M Mallar Chakravarty; Wei-Tang Chang; Marc Dhenain; Dominik von Elverfeldt; Laura-Adela Harsan; Andreas Hess; Tianzi Jiang; Georgios A Keliris; Jason P Lerch; Andreas Meyer-Lindenberg; Hideyuki Okano; Markus Rudin; Alexander Sartorius; Annemie Van der Linden; Marleen Verhoye; Wolfgang Weber-Fahr; Nicole Wenderoth; Valerio Zerbi; Alessandro Gozzi Journal: Neuroimage Date: 2019-10-12 Impact factor: 6.556
Authors: Jaymin Upadhyay; Christian Geber; Richard Hargreaves; Frank Birklein; David Borsook Journal: Neurosci Biobehav Rev Date: 2017-08-12 Impact factor: 8.989
Authors: Luis M Colon-Perez; Kelvin Tran; Khalil Thompson; Michael C Pace; Kenneth Blum; Bruce A Goldberger; Mark S Gold; Adriaan W Bruijnzeel; Barry Setlow; Marcelo Febo Journal: Neuropsychopharmacology Date: 2016-03-21 Impact factor: 7.853
Authors: Brian D Mills; David S Grayson; Anandakumar Shunmugavel; Oscar Miranda-Dominguez; Eric Feczko; Eric Earl; Kim A Neve; Damien A Fair Journal: J Neurosci Date: 2018-05-22 Impact factor: 6.167