OBJECTIVE: The aim of this study is to validate and investigate the clinical value of urinary enolase I in patients with endometriosis. METHODS: Urine samples of 39 patients with histologically confirmed endometriosis and 20 patients without endometriosis were collected. Western blot analysis and enzyme-linked immunosorbent assay were used to detect the increase of enolase I in patients' urine. RESULTS: Urinary enolase I expression corrected for creatinine ratio (non neuronal enolase (NNE)-Cr) was significantly greater in patients with endometriosis (p = 0.026). When the diagnostic performance of NNE-Cr was evaluated with serum CA-125 combination, the area under the curve was 0.821 (95% confidence interval 0.713-0.928) with sensitivity and specificity of 76.9% and 85.0%, respectively. CONCLUSION: Elevated urinary enolase I, in conjunction with serum CA-125, may be used as a potential biomarker for endometriosis.
OBJECTIVE: The aim of this study is to validate and investigate the clinical value of urinary enolase I in patients with endometriosis. METHODS: Urine samples of 39 patients with histologically confirmed endometriosis and 20 patients without endometriosis were collected. Western blot analysis and enzyme-linked immunosorbent assay were used to detect the increase of enolase I in patients' urine. RESULTS: Urinary enolase I expression corrected for creatinine ratio (non neuronal enolase (NNE)-Cr) was significantly greater in patients with endometriosis (p = 0.026). When the diagnostic performance of NNE-Cr was evaluated with serum CA-125 combination, the area under the curve was 0.821 (95% confidence interval 0.713-0.928) with sensitivity and specificity of 76.9% and 85.0%, respectively. CONCLUSION: Elevated urinary enolase I, in conjunction with serum CA-125, may be used as a potential biomarker for endometriosis.
Authors: Emily Liu; Vicki Nisenblat; Cindy Farquhar; Ian Fraser; Patrick M M Bossuyt; Neil Johnson; M Louise Hull Journal: Cochrane Database Syst Rev Date: 2015-12-23
Authors: Vicki Nisenblat; Lucy Prentice; Patrick M M Bossuyt; Cindy Farquhar; M Louise Hull; Neil Johnson Journal: Cochrane Database Syst Rev Date: 2016-07-13
Authors: Katherine E Williams; Olga Miroshnychenko; Eric B Johansen; Richard K Niles; Rajeshwari Sundaram; Kurunthachalam Kannan; Matthew Albertolle; Yan Zhou; Namrata Prasad; Penelope M Drake; Linda C Giudice; Steven C Hall; H Ewa Witkowska; Germaine M Buck Louis; Susan J Fisher Journal: J Proteomics Date: 2014-10-13 Impact factor: 4.044