Literature DB >> 2428160

Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis.

G Wensvoort, C Terpstra, J Boonstra, M Bloemraad, D Van Zaane.   

Abstract

Thirteen hybridoma cell lines which secrete monoclonal antibodies (MCAs) against swine fever virus (SFV) strain Brescia were produced. The hybrid cells resulted from fusion of P3X63-Ag8.653 myeloma cells with splenocytes of Balb/c mice which had been immunized with purified SFV. Screening of supernatant fluids was performed by an indirect enzyme-linked immunosorbent assay (ELISA) and an immunoperoxidase monolayer assay (IPMA). The IPMA, and an immunoperoxidase test (IPT) performed on cryostat sections, were used to characterize these MCAs on several pestivirus strains. All MCAs reacted to a varying degree with all but one of the SFV strains tested. None of the MCAs reacted with the bovine viral diarrhoea virus (BVDV) strains. Two MCAs are now used routinely in the differential diagnosis between infections with field strains of SFV, and the Chinese vaccine strain and BVDV strains.

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Year:  1986        PMID： 2428160     DOI： 10.1016/0378-1135(86)90072-6

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  43 in total

1.  Posttranslational processing and identification of a neutralization domain of the GP4 protein encoded by ORF4 of Lelystad virus.

Authors:  J J Meulenberg; A P van Nieuwstadt; A van Essen-Zandbergen; J P Langeveld
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

2.  Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain).

Authors:  A Caij; G Muyldermans; A De Smet; R Hamers; F Koenen
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

3.  An internal duplication in the 5' noncoding region of strain H: a bovine viral diarrhoea virus (BVDV) isolated from pigs.

Authors:  H G van Gennip; M N Widjojoatmodjo; A J de Smit; R J Moormann
Journal:  Virus Genes       Date:  1999       Impact factor: 2.332

4.  Infectious bursal disease virus capsid protein VP3 interacts both with VP1, the RNA-dependent RNA polymerase, and with viral double-stranded RNA.

Authors:  Mirriam G J Tacken; Ben P H Peeters; Adri A M Thomas; Peter J M Rottier; Hein J Boot
Journal:  J Virol       Date:  2002-11       Impact factor: 5.103

5.  Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence.

Authors:  B P Peeters; O S de Leeuw; G Koch; A L Gielkens
Journal:  J Virol       Date:  1999-06       Impact factor: 5.103

6.  Border disease virus: delineation by monoclonal antibodies.

Authors:  D J Paton; J J Sands; S Edwards
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

7.  Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera.

Authors:  M M Hulst; D F Westra; G Wensvoort; R J Moormann
Journal:  J Virol       Date:  1993-09       Impact factor: 5.103

8.  Bovine respiratory syncytial virus replicates minimally in bovine alveolar macrophages.

Authors:  R S Schrijver; J A Kramps; W G Middel; J P Langedijk; J T van Oirschot
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

9.  Nucleocapsid protein N of Lelystad virus: expression by recombinant baculovirus, immunological properties, and suitability for detection of serum antibodies.

Authors:  J J Meulenberg; R J Bende; J M Pol; G Wensvoort; R J Moormann
Journal:  Clin Diagn Lab Immunol       Date:  1995-11

10.  Live attenuated pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus protects swine against both pseudorabies and hog cholera.

Authors:  M van Zijl; G Wensvoort; E de Kluyver; M Hulst; H van der Gulden; A Gielkens; A Berns; R Moormann
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103
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