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Literature DB >> 24275869

Synthesis of acyl-CoAs by isolated spinach chloroplasts in relation to added CoA and ATP.

Abstract

The kinetics of incorporation of [2-(14)C] acetate into lipids and acyl-CoAs in relation to added CoA and ATP by isolated spinach chloroplasts have been examined. The effect of the concentration of these cofactors on lipid and acyl-CoA synthesis was also studied. In the absence of cofactors, or when only one was present, the incorporation was very low and went mainly into lipids. When both cofactors were present a strong stimulation of both activities occurred. After 25 min, acyl-CoAs were more strongly labeled than lipids and both activities continued linearly for at least 60 min.

Entities: Chemical Species

Year: 1981 PMID： 24275869 DOI： 10.1007/BF00385535

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

14 in total

1. Fat metabolism in higher plants. The function of acyl thioesterases in the metabolism of acyl-coenzymes A and acyl-acyl carrier proteins.

Authors: W E Shine; M Mancha; P K Stumpf
Journal: Arch Biochem Biophys Date: 1976-01 Impact factor: 4.013

2. A comment on the spectrophotometric determination of chlorophyll.

Authors: J BRUINSMA
Journal: Biochim Biophys Acta Date: 1961-09-30

3. Characterization of an acyl-coenzyme a thioesterase associated with the envelope of spinach chloroplasts.

Authors: J Joyard; P K Stumpf
Journal: Plant Physiol Date: 1980-06 Impact factor: 8.340

4. Some properties of a microsomal oleate desaturase from leaves.

Authors: C R Slack; P G Roughan; J Terpstra
Journal: Biochem J Date: 1976-04-01 Impact factor: 3.857

5. Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope.

Authors: P G Roughan; C R Slack
Journal: Biochem J Date: 1977-02-15 Impact factor: 3.857

6. Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea.

Authors: J B Ohlrogge; D N Kuhn; P K Stumpf
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

7. The kinetics of incorporation in vivo of (14C)acetate and (14C)carbon dioxide into the fatty acids of glycerolipids in developing leaves.

Authors: C R Slack; P G Roughan
Journal: Biochem J Date: 1975-11 Impact factor: 3.857

Synthesis of acyl-CoAs by isolated spinach chloroplasts in relation to added CoA and ATP.

1. Fat metabolism in higher plants. The function of acyl thioesterases in the metabolism of acyl-coenzymes A and acyl-acyl carrier proteins.

2. A comment on the spectrophotometric determination of chlorophyll.

3. Characterization of an acyl-coenzyme a thioesterase associated with the envelope of spinach chloroplasts.

4. Some properties of a microsomal oleate desaturase from leaves.

5. Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope.

6. Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea.

7. The kinetics of incorporation in vivo of (14C)acetate and (14C)carbon dioxide into the fatty acids of glycerolipids in developing leaves.

8. Linoleate and alpha-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts.

9. Site of synthesis of phosphatidic acid and diacyglycerol in spinach chloroplasts.

10. High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts.

1. Subcellular localization of triacylglycerol synthesis in spinach leaves.

2. A role for diacylglycerol acyltransferase during leaf senescence.

3. Grass lignin acylation: p-coumaroyl transferase activity and cell wall characteristics of C3 and C4 grasses.