Laurent Dortet1, Gaelle Cuzon, Patrice Nordmann. 1. INSERM U914 'Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France.
Abstract
OBJECTIVES: Carbapenem-resistant Enterobacteriaceae isolates (n = 1485) were received at the French Associated National Reference Center for Antibiotic Resistance in 2012 and were characterized for their mechanism of resistance to carbapenems. METHODS: Carbapenemase production was detected using the biochemical-based Carba NP test, based on the detection of in vitro hydrolysis of imipenem. All isolates with a positive Carba NP test result were characterized by PCR and sequencing. RESULTS: Carbapenemase production was identified in 23.1% of the isolates. The main carbapenemase type identified was OXA-48 and derivatives (75.5%). An overseas source was clearly demonstrated for only 27.6% of the isolates. CONCLUSIONS: OXA-48 and derivatives are now the most prevalent carbapenemases in France, with a possible spread of these producers in the community.
OBJECTIVES:Carbapenem-resistant Enterobacteriaceae isolates (n = 1485) were received at the French Associated National Reference Center for Antibiotic Resistance in 2012 and were characterized for their mechanism of resistance to carbapenems. METHODS: Carbapenemase production was detected using the biochemical-based Carba NP test, based on the detection of in vitro hydrolysis of imipenem. All isolates with a positive Carba NP test result were characterized by PCR and sequencing. RESULTS: Carbapenemase production was identified in 23.1% of the isolates. The main carbapenemase type identified was OXA-48 and derivatives (75.5%). An overseas source was clearly demonstrated for only 27.6% of the isolates. CONCLUSIONS: OXA-48 and derivatives are now the most prevalent carbapenemases in France, with a possible spread of these producers in the community.
Entities:
Keywords:
Carba NP test; K. pneumoniae; NDM; OXA-48; antibiotics; resistance
Authors: Jesús Oteo; Adriana Ortega; Rosa Bartolomé; Germán Bou; Carmen Conejo; Marta Fernández-Martínez; Juan José González-López; Laura Martínez-García; Luis Martínez-Martínez; María Merino; Elisenda Miró; Marta Mora; Ferran Navarro; Antonio Oliver; Álvaro Pascual; Jesús Rodríguez-Baño; Guillermo Ruiz-Carrascoso; Patricia Ruiz-Garbajosa; Laura Zamorano; Verónica Bautista; María Pérez-Vázquez; José Campos Journal: Antimicrob Agents Chemother Date: 2015-03-30 Impact factor: 5.191
Authors: Juan A Vallejo; Marta Martínez-Guitián; Juan C Vázquez-Ucha; Concepción González-Bello; Margarita Poza; John D Buynak; Christopher R Bethel; Robert A Bonomo; German Bou; Alejandro Beceiro Journal: J Antimicrob Chemother Date: 2016-04-28 Impact factor: 5.790