Literature DB >> 2427486

The application of lipid-soluble stains in plastic-embedded sections.

H van Goor, P O Gerrits, J Grond.   

Abstract

The present study was designed to develop a routine method for direct demonstration and precise localization of lipid substances in tissue sections. A panel of lipid-rich tissues was fixed in 4% buffered formaldehyde, infiltrated, and embedded in the water-soluble plastics Technovit 7100, EFL-67, and JB-4. The use of alcohol containing fluids was avoided. Staining with the lipid-soluble dyes Sudan Black B and Oil Red O revealed excellent preservation of tissue lipids in Technovit 7100 embedded sections when compared with cryostat sections of the same tissue specimens. Lipid preservation in EFL-67 and JB-4 embedded sections was inconsistent, even when infiltration and polymerization procedures were performed at 4 degrees C. Combination of lipid-soluble dyes with the periodic acid Schiff, Jones' methenamine silver, or Gomori' reticulin method allowed for an exact localization of lipids in high-quality Technovit 7100 embedded sections. The procedure herein is easily applicable in routine histopathology practice.

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Year:  1986        PMID： 2427486     DOI： 10.1007/bf00494811

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  7 in total

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Journal:  Beitr Pathol       Date:  1972

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Authors:  G H Cope; M A Williams
Journal:  J Microsc       Date:  1969       Impact factor: 1.758

3.  Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy. I. Glutaraldehyde, osmium tetroxide, Araldite methods.

Authors:  G H Cope; M A Williams
Journal:  J Microsc       Date:  1969       Impact factor: 1.758

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Journal:  Arch Pathol       Date:  1966-05

5.  An embedding method for histochemical studies of undecalcified skeletal growth plate.

Authors:  W A Horton; N Dockery; D Sillence; D L Rimoin
Journal:  Stain Technol       Date:  1980-01

6.  The influence of dehydration media and catalyst systems upon the enzyme activity of tissues embedded in 2-hydroxyethyl methacrylate: an evaluation of three dehydration media and two catalyst systems.

Authors:  P O Gerrits; R Zuideveld
Journal:  Mikroskopie       Date:  1983-12

7.  A new, less toxic polymerization system for the embedding of soft tissues in glycol methacrylate and subsequent preparing of serial sections.

Authors:  P O Gerrits; L Smid
Journal:  J Microsc       Date:  1983-10       Impact factor: 1.758
  7 in total
  13 in total

1.  Digital analysis of hepatic sections in mice accurately quantitates triglycerides and selected properties of lipid droplets.

Authors:  Fengxia Ge; Harrison Lobdell; Shengli Zhou; Chunguang Hu; Paul D Berk
Journal:  Exp Biol Med (Maywood)       Date:  2010-10-13

2.  Chemical mapping of the colorectal cancer microenvironment via MALDI imaging mass spectrometry (MALDI-MSI) reveals novel cancer-associated field effects.

Authors:  R Mirnezami; K Spagou; P A Vorkas; M R Lewis; J Kinross; E Want; H Shion; R D Goldin; A Darzi; Z Takats; E Holmes; O Cloarec; J K Nicholson
Journal:  Mol Oncol       Date:  2013-09-14       Impact factor: 6.603

3.  C/EBPbeta, when expressed from the C/ebpalpha gene locus, can functionally replace C/EBPalpha in liver but not in adipose tissue.

Authors:  S S Chen; J F Chen; P F Johnson; V Muppala; Y H Lee
Journal:  Mol Cell Biol       Date:  2000-10       Impact factor: 4.272

4.  Metformin prevents alcohol-induced liver injury in the mouse: Critical role of plasminogen activator inhibitor-1.

Authors:  Ina Bergheim; Luping Guo; Molly Anne Davis; Jason C Lambert; Juliane I Beier; Ilinca Duveau; James P Luyendyk; Robert A Roth; Gavin E Arteel
Journal:  Gastroenterology       Date:  2006-06       Impact factor: 22.682

5.  MALDI imaging MS of phospholipids in the mouse lung.

Authors:  Karin A Zemski Berry; Bilan Li; Susan D Reynolds; Robert M Barkley; Miguel A Gijón; Joseph A Hankin; Peter M Henson; Robert C Murphy
Journal:  J Lipid Res       Date:  2011-04-20       Impact factor: 5.922

6.  Daughters of the Enamel Organ: Development, Fate, and Function of the Stratum Intermedium, Stellate Reticulum, and Outer Enamel Epithelium.

Authors:  Hui Liu; Xiulin Yan; Mirali Pandya; Xianghong Luan; Thomas G H Diekwisch
Journal:  Stem Cells Dev       Date:  2016-09-09       Impact factor: 3.272

7.  Subhepatotoxic exposure to arsenic enhances lipopolysaccharide-induced liver injury in mice.

Authors:  Gavin E Arteel; Luping Guo; Thomas Schlierf; Juliane I Beier; J Phillip Kaiser; Theresa S Chen; Marsha Liu; Daniel J Conklin; Heather L Miller; Claudia von Montfort; J Christopher States
Journal:  Toxicol Appl Pharmacol       Date:  2007-08-31       Impact factor: 4.219

8.  Towards lipidomics of low-abundant species for exploring tumor heterogeneity guided by high-resolution mass spectrometry imaging.

Authors:  Jonathan Cimino; David Calligaris; Johann Far; Delphine Debois; Silvia Blacher; Nor Eddine Sounni; Agnès Noel; Edwin De Pauw
Journal:  Int J Mol Sci       Date:  2013-12-17       Impact factor: 5.923

9.  Effect of Lactobacillus plantarum FH185 on the Reduction of Adipocyte Size and Gut Microbial Changes in Mice with Diet-induced Obesity.

Authors:  Sun-Young Park; Seong-A Cho; Myung-Ki Lee; Sang-Dong Lim
Journal:  Korean J Food Sci Anim Resour       Date:  2015-04-30       Impact factor: 2.622

10.  Anti-obesity Effect of Yogurt Fermented by Lactobacillus plantarum Q180 in Diet-induced Obese Rats.

Authors:  Sun-Young Park; Ki-Seung Seong; Sang-Dong Lim
Journal:  Korean J Food Sci Anim Resour       Date:  2016-02-28       Impact factor: 2.622
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