| Literature DB >> 24274513 |
Xuejing Jia1, Chunbang Ding, Shu Yuan, Zhongwei Zhang, Yang'er Chen, Lei Du, Ming Yuan.
Abstract
In the present study, the extraction, purification and characterization of polysaccharides from Hawk mature leaf tea (HMP) were investigated. The optimal extraction parameters were obtained by using a Box-Behnken design as follows: extraction temperature 88.9 °C, extraction time 128.2 min and ratio of water to solid 11.4 mL/g. The crude HMP was sequentially purified by chromatography of DEAE-52, and two purified fractions, HMP-1 and HMP-2, were obtained. HMP-1 and HMP-2 were mainly composed of arabinose, galactose, glucose and mannose with the molecular weight of 133 and 100 kDa, respectively. For antioxidant activities in vitro, HMP-1 had strong 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing activity power (FRAP). These results provide a scientific basis for the further use of polysaccharides from this traditional herb tea.Entities:
Keywords: Antioxidant activity; Litsea coreana var. lanuginose; Polysaccharide; Response surface methodology
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Year: 2013 PMID： 24274513 DOI： 10.1016/j.carbpol.2013.07.090
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381