How ultrasound technologies have expanded and revolutionized research in reproduction in large animals.

Gray-scale ultrasonic imaging (UI) was introduced in 1980 and initially was used to examine clinically the reproductive tract of mares. By 1983 in mares and 1984 in heifers/cows, UI had become a tool for basic research. In each species, transrectal gray-scale UI has been used extensively to characterize follicle dynamics and investigate the gonadotropic control and hormonal role of the follicles. However, the use of transrectal UI has also disclosed and characterized many other aspects of reproduction in each species, including (1) endometrial echotexture as a biological indicator of circulating estradiol concentrations, (2) relative location of the genital tubercle for fetal gender diagnosis by Days 50 to 60, and (3) timing of follicle evacuation during ovulation. Discoveries in mares include (1) embryo mobility wherein the spherical conceptus (6-16 mm) travels to all parts of the uterus on Days 11 to 15, (2) how one embryo of a twin set eliminates the other without self-inflicted damage, and (3) serration of the granulosum of the preovulatory follicle opposite to the future rupture site as an indicator of imminent ovulation. Studies with color-Doppler UI have shown that vascular perfusion of the endometrium follows the equine embryo back and forth between uterine horns and follows the expansion of the bovine allantochorion throughout each horn. In heifers, blood flow in the CL increases during the ascending portion of an individual pulse of PGF2α metabolite and then decreases. These examples highlight the power of UI in reproduction research. Without UI, it is likely that these and many other findings would still be unknown.