OBJECTIVES: This study aimed to document the association of human papilloma virus (HPV) and its types in breast carcinoma tissues in Kuwaiti women, and correlate this with known prognostic markers. METHODS: The clinicopathological data of archived tissue from 144 cases of invasive ductal breast carcinoma were studied (age, histological grade, size of tumour, lymph node metastases, oestrogen/progesterone receptors and human epidermal growth factor receptor 2 status). HPV frequency was documented using immunohistochemistry (IHC) and chromogenic in-situ hybridisation (CISH). HPV types were documented by CISH using HPV probes. CISH and IHC techniques were compared and HPV correlated with prognostic parameters. RESULTS: The HPV prevalence as determined by CISH and IHC was 51 (35.4%) and 24 (16.7%) cases, respectively. The sensitivity of HPV by IHC was 37.3% and specificity was 94.6%. The sensitivity and specificity of HPV-CISH compared to HPVIHC was statistically significant (P <0.001). HPV-CISH was seen in 51 cases. A combination of HPV 6 and 11, and 16 and 18 was seen in 2 (3.9%) cases, and a combination of HPV 6, 11, 31 and 33 was seen in 7 (13.7%) cases. All three HPV probes: 6 and 11, 16 and 18, as well as 31 and 33 were present in 2 (3.9%) cases. The prevalence of HPVCISH in the Kuwaiti and non-Kuwaiti populations was 27 (52.9%) and 19 (37.2%), respectively. No correlation was observed with the prognostic parameters. CONCLUSION: The frequency of HPV in breast carcinoma cases in Kuwait was 35.4% (CISH). Of those, 52.9% were Kuwaitis in whom both low- and high-risk HPV types were detected.
OBJECTIVES: This study aimed to document the association of human papilloma virus (HPV) and its types in breast carcinoma tissues in Kuwaiti women, and correlate this with known prognostic markers. METHODS: The clinicopathological data of archived tissue from 144 cases of invasive ductal breast carcinoma were studied (age, histological grade, size of tumour, lymph node metastases, oestrogen/progesterone receptors and humanepidermal growth factor receptor 2 status). HPV frequency was documented using immunohistochemistry (IHC) and chromogenic in-situ hybridisation (CISH). HPV types were documented by CISH using HPV probes. CISH and IHC techniques were compared and HPV correlated with prognostic parameters. RESULTS: The HPV prevalence as determined by CISH and IHC was 51 (35.4%) and 24 (16.7%) cases, respectively. The sensitivity of HPV by IHC was 37.3% and specificity was 94.6%. The sensitivity and specificity of HPV-CISH compared to HPVIHC was statistically significant (P <0.001). HPV-CISH was seen in 51 cases. A combination of HPV 6 and 11, and 16 and 18 was seen in 2 (3.9%) cases, and a combination of HPV 6, 11, 31 and 33 was seen in 7 (13.7%) cases. All three HPV probes: 6 and 11, 16 and 18, as well as 31 and 33 were present in 2 (3.9%) cases. The prevalence of HPVCISH in the Kuwaiti and non-Kuwaiti populations was 27 (52.9%) and 19 (37.2%), respectively. No correlation was observed with the prognostic parameters. CONCLUSION: The frequency of HPV in breast carcinoma cases in Kuwait was 35.4% (CISH). Of those, 52.9% were Kuwaitis in whom both low- and high-risk HPV types were detected.
Entities:
Keywords:
Breast Neoplasm; Human Papillomavirus; Immunohistochemistry; In Situ Hybridization; Kuwait
Authors: Kimberly Baltzell; Gertrude C Buehring; Savitri Krishnamurthy; Henry Kuerer; Hua Min Shen; Jennette D Sison Journal: Cancer Date: 2011-08-05 Impact factor: 6.860
Authors: Mohamed Motawy; Omar El Hattab; Salah Fayaz; Medhat Oteifa; Juzer Ali; Thomas George; Ibrahim Barghash; Sadeq Abuzallouf; Mohammed El Jarallah Journal: J Egypt Natl Canc Inst Date: 2004-06
Authors: M Gumus; P F Yumuk; T Salepci; M Aliustaoglu; F Dane; M Ekenel; G Basaran; H Kaya; N Barisik; N S Turhal Journal: J Exp Clin Cancer Res Date: 2006-12
Authors: Y Liu; V S Klimberg; N R Andrews; C R Hicks; H Peng; M Chiriva-Internati; R Henry-Tillman; P L Hermonat Journal: J Hum Virol Date: 2001 Nov-Dec
Authors: Ethel-Michele de Villiers; Robert E Sandstrom; Harald zur Hausen; Charles E Buck Journal: Breast Cancer Res Date: 2004-10-22 Impact factor: 6.466
Authors: N A Khan; A Castillo; C Koriyama; Y Kijima; Y Umekita; Y Ohi; M Higashi; Y Sagara; H Yoshinaka; T Tsuji; S Natsugoe; T Douchi; Y Eizuru; S Akiba Journal: Br J Cancer Date: 2008-07-22 Impact factor: 7.640
Authors: Paolo Fortina; Najib Al Khaja; Mahmoud Taleb Al Ali; Abdul Rezzak Hamzeh; Pratibha Nair; Federico Innocenti; George P Patrinos; Larry J Kricka Journal: Hum Mutat Date: 2014-03-31 Impact factor: 4.878
Authors: Abdallah Mohammed Elagali; Ahmed Abdelbadie Suliman; Mohammed Altayeb; Anas Ibrahim Dannoun; Narasimha Reddy Parine; Hader Ibrahim Sakr; Howayda Saeed Suliman; Moustafa Elsaeid Motawee Journal: Pan Afr Med J Date: 2021-01-15
Authors: Nabila El-Sheikh; Nahla O Mousa; Amany M Tawfeik; Alaa M Saleh; Iman Elshikh; Mohamed Deyab; Faten Ragheb; Manar M Moneer; Ahmed Kawashti; Ahmed Osman; Mohamed Elrefaei Journal: Breast Cancer (Auckl) Date: 2021-02-25