OBJECTIVES: Pompe disease (glycogen storage disease type II) is a rare autosomal recessive lysosomal storage disease that is caused by acid alpha-glucosidase deficiency. Early enzyme replacement therapy can benefit infants with the disease but the diagnosis is complicated by the rarity of the disease and the heterogeneity of the clinical manifestations. In this study, DNA extracted from archival postmortem formalin-fixed paraffin-embedded tissues was used to identify Pompe disease mutations in Oman and develop a rapid molecular-based test. METHODS: Intronic primers were designed to amplify short fragments (193-454 base pairs [bp]) from coding exons (2-20) and screen for mutations using direct sequencing (DS). RESULTS: Two mutations known to cause severe disease were identified in two samples. One was a coding mutation, c.2560C>T (p.Arg854X), and the second was found at a splice acceptor site, c.1327-2A>G. Polymerase chain reaction- and restriction fragment length polymorphism-based tests were designed for the rapid genotyping of the identified mutations. CONCLUSION: These tests can facilitate prenatal diagnosis and help in identifying carriers in families with the identified mutations.
OBJECTIVES: Pompe disease (glycogen storage disease type II) is a rare autosomal recessive lysosomal storage disease that is caused by acid alpha-glucosidase deficiency. Early enzyme replacement therapy can benefit infants with the disease but the diagnosis is complicated by the rarity of the disease and the heterogeneity of the clinical manifestations. In this study, DNA extracted from archival postmortem formalin-fixed paraffin-embedded tissues was used to identify Pompe disease mutations in Oman and develop a rapid molecular-based test. METHODS: Intronic primers were designed to amplify short fragments (193-454 base pairs [bp]) from coding exons (2-20) and screen for mutations using direct sequencing (DS). RESULTS: Two mutations known to cause severe disease were identified in two samples. One was a coding mutation, c.2560C>T (p.Arg854X), and the second was found at a splice acceptor site, c.1327-2A>G. Polymerase chain reaction- and restriction fragment length polymorphism-based tests were designed for the rapid genotyping of the identified mutations. CONCLUSION: These tests can facilitate prenatal diagnosis and help in identifying carriers in families with the identified mutations.
Authors: Marian Kroos; Marianne Hoogeveen-Westerveld; Helen Michelakakis; Robert Pomponio; Ans Van der Ploeg; Dicky Halley; Arnold Reuser Journal: Hum Mutat Date: 2012-05-29 Impact factor: 4.878
Authors: Marian Kroos; Robert J Pomponio; Laura van Vliet; Rachel E Palmer; Michael Phipps; Robert Van der Helm; Dicky Halley; Arnold Reuser Journal: Hum Mutat Date: 2008-06 Impact factor: 4.878
Authors: Monique M P Hermans; Dik van Leenen; Marian A Kroos; Clare E Beesley; Ans T Van Der Ploeg; Hitoshi Sakuraba; Ron Wevers; Wim Kleijer; Helen Michelakakis; Edwin P Kirk; Janice Fletcher; Nils Bosshard; Lina Basel-Vanagaite; Guy Besley; Arnold J J Reuser Journal: Hum Mutat Date: 2004-01 Impact factor: 4.878
Authors: Priya S Kishnani; Robert D Steiner; Deeksha Bali; Kenneth Berger; Barry J Byrne; Laura E Case; Laura Case; John F Crowley; Steven Downs; R Rodney Howell; Richard M Kravitz; Joanne Mackey; Deborah Marsden; Anna Maria Martins; David S Millington; Marc Nicolino; Gwen O'Grady; Marc C Patterson; David M Rapoport; Alfred Slonim; Carolyn T Spencer; Cynthia J Tifft; Michael S Watson Journal: Genet Med Date: 2006-05 Impact factor: 8.822