| Literature DB >> 24272738 |
W Behrends1, U Rausch, H G Löffler, H Kindl.
Abstract
Glycollate oxidase (glycollate: oxygen oxidoreductase, EC 1.1.3.1) was purified to apparent homogeneity from crude extracts of greening cucumber cotyledons (Cucumis sat vus). Molecular sieving and chromatofocusing resulted in 700-fold purification and specific activity of 1 μkat mg(-1) protein. The enzyme exhibited a Mr of 180,000, or 700,000, respectively, and is a tetramer or 16-mer made of identical subunits of Mr 43,000. Monospecific antibodies were raised against the homogeneous protein.Entities:
Year: 1982 PMID: 24272738 DOI: 10.1007/BF00392782
Source DB: PubMed Journal: Planta ISSN: 0032-0935 Impact factor: 4.116