Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase.

In the present paper we describe the synthesis of Sepharose-boundN-(5'-phosphopyridoxyl)-amino-oxyacetic acid,N-(5'-phosphopyridoxyl)-canaline, andN-(5'-phosphopyridoxyl)-α,γ-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). The three Sepharose-N-(phosphopyridoxyl)-amino acids were capable of binding GAD from a mouse brain soluble preparation. The enzyme can be purified up to 25-fold in one step by affinity chromatography on Seph-DAB-PLP.