Use of ion-pairing reversed-phase liquid chromatography in separation of solanidine and solasodine.

A method based on ion-pairing reversed-phase liquid chromatography was developed to separate and quantify the total alkaloid content inS. ptycanthum (eastern black nightshade). The method is shown to separate satisfactorily the aglycones and some of the glycoalkaloids. The mobile phase used was aqueous sodium dodecyl sulfate-acetonitrile.