Literature DB >> 24271519

Determination of β-Endorphin and Fragments Thereof in Human Plasma Using High-Performance Liquid Chromatography and a Multiple Radioimmunoassay System.

K Wiedemann1, H Teschemacher.   

Abstract

A method for the determination of β-endorphin and β-endorphin fragments in human plasma was developed. β-Endorphin-related peptides were extracted from plasma using octadecasilyl-silica cartridges. Extracts were subjected to re versed-phase high-performance liquid chromatography (HPLC). Extracts as well as HPLC column eluates were assayed using a multiple radioimmunoassay system; several antibodies directed against various distinct regions of the β-endorphin molecule were employed. Using this method, evidence for the presence of multiple β-endorphin fragments in the plasma of healthy young volunteers (under normal conditions) was obtained.

Entities:  

Year:  1986        PMID： 24271519     DOI： 10.1023/A:1016357806879

Source DB:  PubMed          Journal:  Pharm Res        ISSN： 0724-8741            Impact factor:   4.200


  25 in total

1.  Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.

Authors:  H P Bennett; A M Hudson; C McMartin; G E Purdon
Journal:  Biochem J       Date:  1977-10-15       Impact factor: 3.857

2.  beta-Endorphin is not detectable in plasma from normal human subjects.

Authors:  T Suda; A S Liotta; D T Krieger
Journal:  Science       Date:  1978-10-13       Impact factor: 47.728

3.  Development and validation of a sensitive radioimmunoassay for naturally occurring beta-endorphin-like peptides in human plasma.

Authors:  V E Ghazarossian; R R Dent; K Otsu; M Ross; B Cox; A Goldstein
Journal:  Anal Biochem       Date:  1980-02       Impact factor: 3.365

4.  Rapid separation of enkephalins and endorphins on Sep-Pak reverse phase cartridges.

Authors:  D D Gay; R A Lahti
Journal:  Int J Pept Protein Res       Date:  1981-07

5.  Antibodies specific for alpha-N-acetyl-beta-endorphins: radioimmunoassays and detection of acetylated beta-endorphins in pituitary extracts.

Authors:  E Weber; C J Evans; J K Chang; J D Barchas
Journal:  J Neurochem       Date:  1982-02       Impact factor: 5.372

6.  Measurement of total opioid peptides in rat brain and pituitary by radioimmunoassay directed at the alpha-N-acetyl derivative.

Authors:  M R Boarder; E Weber; C J Evans; E Erdelyi; J Barchas
Journal:  J Neurochem       Date:  1983-06       Impact factor: 5.372

Review 7.  Distribution of beta-endorphin-related peptides in rat pituitary and brain.

Authors:  S Zakarian; D G Smyth
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

8.  High-pressure liquid chromatography and radioimmunoassay for the specific and quantitative determination of endorphins and related peptides.

Authors:  J G Loeber; J Verhoef
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

9.  Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane-bonded silica.

Authors:  M J O'Hare; E C Nice
Journal:  J Chromatogr       Date:  1979-04-01

10.  beta-Endorphin immunoreactive materials in human plasma determined by a multiple radioimmunoassay system.

Authors:  K Wiedemann; H Teschemacher
Journal:  Life Sci       Date:  1983       Impact factor: 5.037
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  1 in total

1.  A biotin-avidin-based enzyme immunoassay for beta h-endorphin.

Authors:  G Hochhaus; W Sadée
Journal:  Pharm Res       Date:  1988-04       Impact factor: 4.200
  1 in total

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