Literature DB >> 24271199

Expression of Anabaena ferredoxin genes in Escherichia coli.

H Böhme1, R Haselkorn.   

Abstract

The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) of Anabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels (≈10% of total protein). Pet F could be expressed from its own promoter. The ferredoxins were correctly assembled to the holoprotein. Heterocyst ferredoxin was purified from E. coli extracts on a large scale. Its biochemical and biophysical properties were identical to those of the authentic ferredoxin, isolated from Anabaena heterocysts.

Entities:  

Year:  1989        PMID： 24271199     DOI： 10.1007/BF00044157

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  11 in total

1.  Cloning, expression, and characterization of the Anabaena thioredoxin gene in Escherichia coli.

Authors:  C J Lim; F K Gleason; J A Fuchs
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

2.  Activity expressed from cloned Anacystis nidulans large and small subunit ribulose bisphosphate carboxylase genes.

Authors:  J T Christeller; B E Terzaghi; D F Hill; W A Laing
Journal:  Plant Mol Biol       Date:  1985-07       Impact factor: 4.076

3.  Molecular cloning and nucleotide sequence analysis of the gene coding for heterocyst ferredoxin from the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  H Böhme; R Haselkorn
Journal:  Mol Gen Genet       Date:  1988-10

4.  Escherichia coli ferredoxin, an iron-sulfur protein of the adrenodoxin type.

Authors:  H E Knoell; J Knappe
Journal:  Eur J Biochem       Date:  1974-12-16

5.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469

6.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Cloning of phosphoenolpyruvate carboxylase gene from a cyanobacterium, Anacystis nidulans, in Escherichia coli.

Authors:  T Kodaki; F Katagiri; M Asano; K Izui; H Katsuki
Journal:  J Biochem       Date:  1985-02       Impact factor: 3.387

8.  Pathway of assembly of ribulosebisphosphate carboxylase/oxygenase from Anabaena 7120 expressed in Escherichia coli.

Authors:  M Gurevitz; C R Somerville; L McIntosh
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

9.  Expression and assembly of active cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli containing stoichiometric amounts of large and small subunits.

Authors:  F R Tabita; C L Small
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

10.  Isolation and sequence of the gene for ferredoxin I from the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  J Alam; R A Whitaker; D W Krogmann; S E Curtis
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490
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  5 in total

1.  Differential activities of heterocyst ferredoxin, vegetative cell ferredoxin, and flavodoxin as electron carriers in nitrogen fixation and photosynthesis in Anabaena sp.

Authors:  P Razquin; S Schmitz; M L Peleato; M F Fillat; C Gómez-Moreno; H Böhme
Journal:  Photosynth Res       Date:  1995-01       Impact factor: 3.573

2.  Identification of the nifJ gene coding for pyruvate: ferredoxin oxidoreductase in dinitrogen-fixing cyanobacteria.

Authors:  O Schmitz; T Kentemich; W Zimmer; B Hundeshagen; H Bothe
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552

3.  Molecular cloning and expression of the gene encoding ADP-glucose pyrophosphorylase from the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  Y Y Charng; G Kakefuda; A A Iglesias; W J Buikema; J Preiss
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

Review 4.  Structure-function studies of [2Fe-2S] ferredoxins.

Authors:  H M Holden; B L Jacobson; J K Hurley; G Tollin; B H Oh; L Skjeldal; Y K Chae; H Cheng; B Xia; J L Markley
Journal:  J Bioenerg Biomembr       Date:  1994-02       Impact factor: 2.945

5.  Evidence from directed mutagenesis that positively charged amino acids are necessary for interaction of nitrogenase with the [2Fe-2S] heterocyst ferredoxin (FdxH) from the cyanobacterium Anabaena sp., PCC7120.

Authors:  S Schmitz; B Schrautemeier; H Böhme
Journal:  Mol Gen Genet       Date:  1993-09
  5 in total

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