INTRODUCTION: [(18)F]fluoroethoxybenzovesamicol ([(18)F]FEOBV) is a PET radiotracer with high selectivity and specificity to the vesicular acetylcholine transporter (VAChT). It has been shown to be a sensitive in vivo measurement of changes of cholinergic innervation densities following lesion of the nucleus basalis of Meynert (NBM) in rat. The current study used [(18)F]FEOBV with PET imaging to detect the effect of a highly selective lesion of the pedunculopontine (PPTg) nucleus in rat. METHODS: After bilateral and selective lesions of the PPTg cholinergic neurons, rats were scanned using [(18)F]FEOBV, then sacrificed, and their brain tissues collected for immunostaining and quantification of the VAChT. RESULTS: Comparisons with control rats revealed that cholinergic losses can be detected in the brainstem, lateral thalamus, and pallidum by using both in vivo imaging methods with [(18)F]FEOBV, and ex vivo measurements. In the brainstem PPTg area, significant correlations were observed between in vivo and ex vivo measurements, while this was not the case in the thalamic and pallidal projection sites. CONCLUSIONS: These findings support PET imaging with [(18)F]FEOBV as a reliable in vivo method for the detection of neuronal terminal losses resulting from lesion of the PPTg. Useful applications can be found in the study of neurodegenerative diseases in human, such as Parkinson's disease, multiple system atrophy, progressive supranuclear palsy, or dementia with Lewy bodies. Crown
INTRODUCTION: [(18)F]fluoroethoxybenzovesamicol ([(18)F]FEOBV) is a PET radiotracer with high selectivity and specificity to the vesicular acetylcholine transporter (VAChT). It has been shown to be a sensitive in vivo measurement of changes of cholinergic innervation densities following lesion of the nucleus basalis of Meynert (NBM) in rat. The current study used [(18)F]FEOBV with PET imaging to detect the effect of a highly selective lesion of the pedunculopontine (PPTg) nucleus in rat. METHODS: After bilateral and selective lesions of the PPTg cholinergic neurons, rats were scanned using [(18)F]FEOBV, then sacrificed, and their brain tissues collected for immunostaining and quantification of the VAChT. RESULTS: Comparisons with control rats revealed that cholinergic losses can be detected in the brainstem, lateral thalamus, and pallidum by using both in vivo imaging methods with [(18)F]FEOBV, and ex vivo measurements. In the brainstem PPTg area, significant correlations were observed between in vivo and ex vivo measurements, while this was not the case in the thalamic and pallidal projection sites. CONCLUSIONS: These findings support PET imaging with [(18)F]FEOBV as a reliable in vivo method for the detection of neuronal terminal losses resulting from lesion of the PPTg. Useful applications can be found in the study of neurodegenerative diseases in human, such as Parkinson's disease, multiple system atrophy, progressive supranuclear palsy, or dementia with Lewy bodies. Crown
Authors: Hongjun Jin; Xuyi Yue; Hui Liu; Junbin Han; Hubert Flores; Yi Su; Stanley M Parsons; Joel S Perlmutter; Zhude Tu Journal: J Neurochem Date: 2018-03-25 Impact factor: 5.372
Authors: Hui Liu; Hongjun Jin; Junfeng Li; Xiang Zhang; Kota Kaneshige; Stanley M Parsons; Joel S Perlmutter; Zhude Tu Journal: Eur J Pharmacol Date: 2015-02-09 Impact factor: 4.432
Authors: Roger L Albin; Prabesh Kanel; Teus van Laar; Sygrid van der Zee; Stiven Roytman; Robert A Koeppe; Peter J H Scott; Nicolaas I Bohnen Journal: Mol Pharm Date: 2022-03-15 Impact factor: 4.939
Authors: Nicolaas I Bohnen; Prabesh Kanel; Zhi Zhou; Robert A Koeppe; Kirk A Frey; William T Dauer; Roger L Albin; Martijn L T M Müller Journal: Ann Neurol Date: 2019-03-13 Impact factor: 10.422
Authors: J Miguel Cisneros-Franco; Patrice Voss; Min Su Kang; Maryse E Thomas; Jonathan Côté; Karen Ross; Pierrette Gaudreau; David A Rudko; Pedro Rosa-Neto; Étienne de-Villers-Sidani Journal: Front Neurosci Date: 2020-01-21 Impact factor: 4.677