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Literature DB >> 24264846

Maintenance of plant cell membrane integrity and function by the immobilisation of protoplasts in alginate matrices.

Abstract

Isolated protoplasts (Vicia faba) suspended in a mannitol solution over a time period of about 10 d undergo a process of aging characterised by increased ethane formation, decrease in oxygen evolution and CO2 uptake, pigment degradation and a change in certain enzyme activities (ribulose-1,5-bisphosphate carboxylase and protease). The technique of immobilisation of protoplasts in a cross-linked alginate matrix offers a way to delay the appearance of the indicators mentioned. Various possible mechanisms for the increased stability of protoplasts are discussed.

Entities: Chemical Species

Year: 1983 PMID： 24264846 DOI： 10.1007/BF00397730

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

17 in total

1. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors: D I Arnon
Journal: Plant Physiol Date: 1949-01 Impact factor: 8.340

2. Pressure probe technique for measuring water relations of cells in higher plants.

Authors: D Hüsken; E Steudle; U Zimmermann
Journal: Plant Physiol Date: 1978-02 Impact factor: 8.340

3. Mechanical stabilization of guard cell protoplasts of Vicia faba.

Authors: H Schnabl; P Scheurich; U Zimmermann
Journal: Planta Date: 1980-08 Impact factor: 4.116

4. Light-dependent ethylene production by isolated chloroplasts.

Authors: E Elstner; J R Konze
Journal: FEBS Lett Date: 1974-09-01 Impact factor: 4.124

5. The stress-strain relation of the plasma membrane of isolated plant protoplasts.

Authors: J Wolfe; P L Steponkus
Journal: Biochim Biophys Acta Date: 1981-05-20

6. Hydrocarbon gases produced during in vitro peroxidation of polyunsaturated fatty acids and decomposition of preformed hydroperoxides.

Authors: E E Dumelin; A L Tappel
Journal: Lipids Date: 1977-11 Impact factor: 1.880

1. Differential effects of electrofusion and electropermeabilization parameters on the membrane integrity of plant protoplasts.

Authors: U Biedinger; R J Youngman; H Schnabl
Journal: Planta Date: 1990-03 Impact factor: 4.116

Maintenance of plant cell membrane integrity and function by the immobilisation of protoplasts in alginate matrices.

1. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

2. Pressure probe technique for measuring water relations of cells in higher plants.

3. Mechanical stabilization of guard cell protoplasts of Vicia faba.

4. Light-dependent ethylene production by isolated chloroplasts.

5. The stress-strain relation of the plasma membrane of isolated plant protoplasts.

6. Hydrocarbon gases produced during in vitro peroxidation of polyunsaturated fatty acids and decomposition of preformed hydroperoxides.

7. Immobilisation and mechanical, support of individual protoplasts.

8. The compartmentation of carboxylating and decarboxylating enzymes in guard cell protoplasts.

9. Water relations of growing pea epicotyl segments.

10. Water relations of immobilized giant algal cells.

1. Differential effects of electrofusion and electropermeabilization parameters on the membrane integrity of plant protoplasts.

2. Improvement of protoplast culture protocols for Beta vulgaris L. (sugar beet).

3. Microcallus growth from maize protoplasts.

4. Plant regeneration from cell suspension-derived protoplasts of Phalaenopsis.