Analysis of inter-relationship of jackbean seed components by two-dimensional mapping of iodinated tryptic peptides.

The subunits of Concanavalin A, α-mannosidase and the main storage protein of jackbeans have been compared using the technique of peptide-mapping of iodinated tryptic peptides. The results indicate there is no structural relatedness between the lectin, enzyme and storage protein. Peptide maps of the lectin fragments which copurify with the intact subunit indicate that cleavage points additional to that between residues 118-119 most probably occur.