A cytogenetical study on Economidichthys pygmaeus Holly, 1929 (Pisces, Gobiidae), an endemic freshwater goby from Western Greece.

A cytogenetic study was carried out on the chromosomes and the nuclear DNA content of the freshwater goby Economidichthys pygmaeus (Pisces, Gobiidae). The species is characterized by a 2n=46 karyotype consisting of 12 submetacentric and 11 subtelocentric chromosome pairs (NF=70). Major (45S) rDNA genes are terminal-centromeric located on the short arm of a single medium-small sized submetacentric pairas assessed by in situ hybridization, CMA3 staining, and Ag-NOR banding. The haploid (C-value) nuclear DNA content is 0.93±0.003 picograms. The cytogenetical data of Economidichthys pygmaeus were compared with those ones already available for other related gobies.

Introduction

is an endemic small-bodied, short-lived species restricted to the north and western part of Greece (Miller 1990). Specifically, it is distributed in the Thiamis, Louros, Arahcthos and Achelloos Rivers and in Lakes Trichonis, Lyssimachia, Ziros and Ozeros. Recently it has been recorded in Lake Pamvotis where it was introduced probably from River Louros and/or Kalamas (Thyamis) (Leonardos et al. 2008). The species appears to be extinct on Lefkas Island (Economidis 1991) and is protected under Greek law No. 67/1981. Greece’s updated edition of the Red Book of Endangered Species (2009) has evaluated its conservation status and it is considered now a ‘least concerned species’, without facing any critical dangers (Crivelli 2006, Economidis 2009). Despite the importance of this species in terms of conservation, information on many aspects of its biology, ecology and behaviour are lacking in the current literature, and no data on karyotype are available at present (Miller 1990). In the aim of promoting the conservation of this species a set of investigations was carried out in order to better understand its biology and life-history. In such research frame the present cytogenetical study is included.

Materials and methods

Ten adult males and five females of were collected from Lake Pamvotis (Ioannina, NW Greece) and used for this study. Animals were injected with colchicine and were killed with an overdose of MS222. After that, the fish were sacrificed and chromosome preparations were obtained from spleen and testis by using the conventional air-drying technique. Chromosome plates were conventionally stained with Giemsa and eventually re-stained with silver nitrate (Howell and Black 1980) to get an Ag-NOR banding. Chromosome classification follows Levan et al. (1964). Mapping of rDNA major complex genes were performed by fluorescent in situ hybridization (FISH) with the pDm238 probe (Roiha et al) containing the 18S-5.8S-28S gene cluster and intergenic spacers of the fruit fly Meigen, 1830, according to the procedure in Libertini et al. (2008). Plates previously analysed by FISH were sequentially stained with chromomycin A3 (CMA3) (Schweizer 1976). Genome size (GS) was assessed by flow cytometry on peripheral erythrocytes, according to the method in Libertini et al. (2003).

Results

The haploid n=23 and the diploid 2n=46 chromosome numbers were determined for (Fig. 1 and Fig. 2) from the counts of 50 and 94 plates, respectively. All the analysed specimens, regardless of sex, shared the same 2n=46 karyotype (Fig. 2), composed of 24 submetacentric (Fig. 2, pairs 1-12) and 22 subtelocentric (PageBreakFig. 2, pairs 13-23) chromosomes. Therefore, the fundamental number of chromosome arms (NF) is 70. Ag-NOR banding (Howell and Black 1980) showed a terminal-centromeric location (following the scheme in Caputo 1998) of the active nucleolar organiser regions (Fig. 2, blue inset) on the short arm in a medium-small sized submetacentric pair (Fig. 1, pair 10). FISH results from first spermatocyte metaphase bivalents (Fig. 3A) and mitotic chromosomes (Fig. 4A) confirmed the previous observations with Ag-NOR banding. In fact, a single bivalent showed hybridization signals with the NORs probe on both ends (Fig. 3A, arrows) in the spermatocyte plates, while in mitotic plates a couple of chromosomes showed rDNA major complex FISH signals on the short arm (Fig. 4A, arrows). CMA3 produced overlapping bright signals in the same location of hybridization signals (Figs 3B and 4B, see arrows), indicating that rDNA major complex gene sequences contain GC-rich DNA. Through flow cytometric essay the GS (haploid C-value) of was evaluated as 0.93±0.003 picograms.

Figure 1.

first spermatocyte metaphase. Bar = 10μm

Figure 2.

karyotype. Pairs 1–12 submetacentrics; pairs 13-23 subtelocentrcs. Inset Ag-NOR staining of pair 10. Bar = 10µm.

Figure 3.

A–B first spermatocyte metaphase A FISH with a rDNA major complex probe B Sequential staining with CMA3.

Figure 4.

A–B mitotic metaphase A FISH with rDNA major complex probe B Sequential staining with CMA3. Bar = 10µm.

Discussion

About one hundred species of Gobiidae have been analysed cytogenetically and show great karyotypic diversity. Diploid number varies from 30 to 62 and variation in chromosome morphology is also wide (NF=40-98). High intraspecific chromosomal variability has been detected in some species (Galetti et al. 2000, and reference therein). In the comparison with the other gobies is characterized by the most common chromosome number (2n=46) and by an unusual karyotype composed exclusively by submetacentric and subtelocentric elements. The resulting NF=70 is intermediate among NF values of Gobiidae (Klinkhardt et al. 1995, and reference therein). The GS of (0.93 pg) is also intermediate among the GS values of the Gobiidae (range 0.42-1.68 pg) (Animal Genome Size Database 2009). A single pair of medium-smallPageBreak chromosomes bearing a GC-rich NOR on a terminal-centromeric zone in the short arm is the most common location of major rDNA genes in Gobiidae, being shared by the genera Linnaeus, 1758, Gill, 1863, Iljin, 1927 and Bianco, Bullock, Miller et Roubal, 1987 (Caputo 1998; present paper; A. Libertini unpublished data), and this location represents probably the plesiomorphic character state (Caputo 1998). The genus along with the genera , Duncker, 1928, and were gathered in the so-called sand goby group (McKay and Miller 1997, Huyse et al. 2004). The sand gobies were clustered as a monophyletic group on morphological (McKay and Miller 1997), molecular (Huyse et al. 2004) and behavioural (Malavasi et al. 2008) grounds. Monophyly of sand gobies is also suggested by the sharing of common, probably plesiomorphic, cytogenetical characters: the chromosome number 2n=46 is present in all the four genera (Klinkhardt et al. 1995, Animal Genome Size Database 2009, present paper), the common NOR location in a terminal-centromeric zone in the short arm in a single submetacentric pair for the genera , and so far studied (Caputo 1998, present paper, Libertini unpublished data), and similar GS values in a narrow range around 1 pg (0.91–1.04 pg) (Animal Genome Size Database 2009, present paper). The wide variability of karyotype formula and NF vsa general constancy of chromosome numbers indicate that non-Robertsonian mechanisms of chromosome rearrangements were more frequently involved in karyotype evolution of the sand gobies.