OBJECTIVE: To investigate the role of mouse nerve growth factor (mNGF) in neutral repair following hypoxic-ischemic brain damage (HIBD) in a neonatal rat model. METHODS: A total of 120 neonatal rats aged 7 days were randomly divided into control group, HIBD group and mNGF group (n=40 /group). Immediately after HIBD, mNGF was intramuscularly injected into rats in the mNGF group. 7, 14, 21 and 28 days after injection, immunohistochemistry and TUNEL staining were performed. RESULTS: In the HIBD group, Nestin expression was mainly found in the CA1, CA2 and CA3 regions of hippocampus. In the mNGF group, Nestin was mainly noted in the DG and CA3 regions. 7, 14 and 28 days after treatment, significant difference was found in the Nestin expression among three groups, but not observed 21 days after treatment. 21 and 28 days after treatment, the number of apoptotic neurons was markedly reduced when compared with that 7 and 14 days after treatment. CONCLUSION: mNGF can improve the neurogenesis and inhibit neural apoptosis in the hippocampus following HIBD in a neonatal rat model.
OBJECTIVE: To investigate the role of mousenerve growth factor (mNGF) in neutral repair following hypoxic-ischemic brain damage (HIBD) in a neonatal rat model. METHODS: A total of 120 neonatal rats aged 7 days were randomly divided into control group, HIBD group and mNGF group (n=40 /group). Immediately after HIBD, mNGF was intramuscularly injected into rats in the mNGF group. 7, 14, 21 and 28 days after injection, immunohistochemistry and TUNEL staining were performed. RESULTS: In the HIBD group, Nestin expression was mainly found in the CA1, CA2 and CA3 regions of hippocampus. In the mNGF group, Nestin was mainly noted in the DG and CA3 regions. 7, 14 and 28 days after treatment, significant difference was found in the Nestin expression among three groups, but not observed 21 days after treatment. 21 and 28 days after treatment, the number of apoptotic neurons was markedly reduced when compared with that 7 and 14 days after treatment. CONCLUSION: mNGF can improve the neurogenesis and inhibit neural apoptosis in the hippocampus following HIBD in a neonatal rat model.
Entities:
Keywords:
Brain; hypoxia/ischemia; nerve growth factor; rat
Authors: Lan Guo; Mason L Yeh; Verginia C Cuzon Carlson; Erin M Johnson-Venkatesh; Hermes H Yeh Journal: J Neurosci Date: 2012-05-30 Impact factor: 6.167
Authors: Peter Riess; Chen Zhang; Kathryn E Saatman; Helmut L Laurer; Luca G Longhi; Ramesh Raghupathi; Philipp M Lenzlinger; Jonathan Lifshitz; John Boockvar; Edmund Neugebauer; Evan Y Snyder; Tracy K McIntosh Journal: Neurosurgery Date: 2002-10 Impact factor: 4.654