In vitro haploid plantlet induction in Physalis ixocarpa brot. through microspore embryogenesis.

Anthers of Physalis ixocarpa Brot. exised from 2-3 mm long flower buds were treated for 2 d at 3°C, and were cultured on the basal medium of NN (1969), supplemented with plant hormones. They formed embryoids from microspores within 6 weeks. Upon transfer to a regeneration medium, embryoids grew into functional plants with the haploid number of chromosomes (n=12).