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Literature DB >> 24258485

Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones.

R D Thompson¹, D Bartels, N P Harberd, R B Flavell.

Abstract

cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A(+) RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.

Entities: Gene Species

Year: 1983 PMID： 24258485 DOI： 10.1007/BF00303930

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

21 in total

1. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin : Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes.

Authors: P I Payne; L M Holt; A J Worland; C N Law
Journal: Theor Appl Genet Date: 1982-06 Impact factor: 5.699

10. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality.

Authors: N G Halford; J M Field; H Blair; P Urwin; K Moore; L Robert; R Thompson; R B Flavell; A S Tatham; P R Shewry
Journal: Theor Appl Genet Date: 1992-01 Impact factor: 5.699

Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones.

1. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin : Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

4. Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA.

5. The organization of the chloroplast DNA in wheat and maize in the region containing the LS gene.

6. Complex organization of zein genes in maize.

7. Cloning and sequence analysis reveal structural variation among related zein genes in maize.

8. Cloning and characterization of ribosomal RNA genes from wheat and barley.

9. Genes and mRNAs coding for zein polypeptides in Zea mays.

10. Isolation and identification of mRNA for the high-molecular-weight storage proteins of wheat endosperm.

1. Restriction fragment analysis of the secalin loci of rye.

2. Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum.

3. Identification of a transposon-like insertion in a Glu-1 allele of wheat.

4. α-amylase genes of wheat are two multigene families which are differentially expressed.

5. The endosperm seed protein Solin: biochemical characterization, induction by ABA and species-specific subunits.

6. RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R.

7. Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat.

8. A genetic map of rye chromosome 1R integrating RFLP and cytogenetic loci.

9. Comparative RFLP-based genetic maps of barley chromosome 5 (1H) and rye chromosome 1R.

10. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality.