Literature DB >> 2425806

Acetylcholine operated ion channel and alpha-bungarotoxin binding site in a human neuroblastoma cell line reside on different molecules.

C Gotti, E Wanke, E Sher, D Fornasari, D Cabrini, F Clementi.   

Abstract

In neurons, alpha-bungarotoxin is often associated with nicotinic receptor but does not always block the acetylcholine operated channel. In a human neuroblastoma cell line, IMR 32, we have demonstrated a large number of alpha-Bungarotoxin binding sites (2640 per cell in non differentiated cells and 4660 per cell in differentiated cells) in presence of 0 to 4 Acetylcholine activated-channels per cell. This neuronal cell line promises to be an useful model for the study of structure and function of the alpha-Bungarotoxin binding site not related to the nicotinic receptor.

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Year:  1986        PMID： 2425806     DOI： 10.1016/0006-291x(86)90344-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Neuronal-type alpha-bungarotoxin receptors and the alpha 5-nicotinic receptor subunit gene are expressed in neuronal and nonneuronal human cell lines.

Authors:  B Chini; F Clementi; N Hukovic; E Sher
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

2.  Purification and characterization of an alpha-bungarotoxin receptor that forms a functional nicotinic channel.

Authors:  C Gotti; A E Ogando; W Hanke; R Schlue; M Moretti; F Clementi
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

3.  Effects of long-term in vitro exposure to aluminum, cadmium or lead on differentiation and cholinergic receptor expression in a human neuroblastoma cell line.

Authors:  C Gotti; D Cabrini; E Sher; F Clementi
Journal:  Cell Biol Toxicol       Date:  1987-12       Impact factor: 6.691

4.  Membrane potential fluorescence: a rapid and highly sensitive assay for nicotinic receptor channel function.

Authors:  Richard W Fitch; Yingxian Xiao; Kenneth J Kellar; John W Daly
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-25       Impact factor: 11.205

5.  Human neuroblastoma cells acquire regulated secretory properties and different sensitivity to Ca2+ and alpha-latrotoxin after exposure to differentiating agents.

Authors:  E Sher; S Denis-Donini; A Zanini; C Bisiani; F Clementi
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539
  5 in total

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