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Literature DB >> 24258041

A new procedure for increasing efficiency of protoplast plating and clone selection.

Abstract

Sea Prep(TM) agarose (FMC corp.) was used as a gelling agent for media in the culture of potato and tomato protoplasts. The concentration and conditions appropriate for gelling and liquifaction were determined. The new method provided increased calli transfer efficiency of up to 50-fold. Shoot formation from the protoplast-derived cells occurred normally and without reduction in frequency.

Entities: Chemical Species

Year: 1983 PMID： 24258041 DOI： 10.1007/BF00270093

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

3 in total

1. Plating of isolated tobacco mesophyll protoplasts on agar medium.

Authors: T Nagata; I Takebe
Journal: Planta Date: 1971-03 Impact factor: 4.116

2. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media.

Authors: K N Kao; M R Michayluk
Journal: Planta Date: 1975-01 Impact factor: 4.116

3. Isolation and Regeneration of Tobacco Mesophyll Cell Protoplasts under Low Osmotic Conditions.

Authors: J F Shepard; R E Totten
Journal: Plant Physiol Date: 1975-04 Impact factor: 8.340

3 in total

11 in total

1. Culture of soybean mesophyll protoplasts in alginate beads.

Authors: D M Tricoli; M B Hein; M G Carnes
Journal: Plant Cell Rep Date: 1986-10 Impact factor: 4.570

2. Relative strengths of the 35S cauliflower mosaic virus, 1', 2', and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue.

Authors: M H Harpster; J A Townsend; J D Jones; J Bedbrook; P Dunsmuir
Journal: Mol Gen Genet Date: 1988-04

3. Nuclear-organelle interaction in Solanum: interspecific cybridizations and their correlation with a plastome dendrogram.

Authors: A Perl; D Aviv; E Galun
Journal: Mol Gen Genet Date: 1991-08

10. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.

Authors: A Perl; D Aviv; E Galun
Journal: Plant Cell Rep Date: 1988-10 Impact factor: 4.570

A new procedure for increasing efficiency of protoplast plating and clone selection.

1. Plating of isolated tobacco mesophyll protoplasts on agar medium.

2. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media.

3. Isolation and Regeneration of Tobacco Mesophyll Cell Protoplasts under Low Osmotic Conditions.

1. Culture of soybean mesophyll protoplasts in alginate beads.

2. Relative strengths of the 35S cauliflower mosaic virus, 1', 2', and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue.

3. Nuclear-organelle interaction in Solanum: interspecific cybridizations and their correlation with a plastome dendrogram.

4. A simple culture method for Brassica hypototyl protoplasts.

5. Immobilization of plant protoplasts: Viability studies.

6. Selection and characterization of cadmium-resistant suspension cultures of the wild tomato Lycopersicon peruvianum.

7. Induction of efficient cell division in alfalfa protoplasts.

8. Sensing of osmotic pressure changes in tomato cells.

9. Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences.

10. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.