| Literature DB >> 24257852 |
T W Baumann1, R Koetz, P Morath.
Abstract
Suspension cultures of Coffea arabica L. are a useful source for methyltransferase preparations of high activity catalysing the transfer of methylgroups from S-adenosyl-L-methionine to 7-methylxanthine and to theobromine producing theobromine and caffeine respectively. Surprisingly, these enzyme activities are not correlated with the availability of precursors during a culture cycle. They are highest in the growth phase when supply of precursors is reduced. Mixed substrate experiments and time dependent changes in the enzyme activity ratio provide indirect evidence for the existence of two separate enzymes catalysing the final methylations in caffeine biosynthesis.Entities:
Year: 1983 PMID: 24257852 DOI: 10.1007/BF00269231
Source DB: PubMed Journal: Plant Cell Rep ISSN: 0721-7714 Impact factor: 4.570