Mutations in rDNA : 3. Modulatory action of α-amanitin on aberration induction in nucleolus organizer regions.

The action of α-amanitin, an inhibitor of RNA synthesis, on the induction by hydroxyurea (HU) of chromosomal aberrations in nucleolus organizer regions of barley was studied. The data obtained show that α-amanitin can effectively modify aberration induction in rDNA. Administered before mutagen treatment or in combination with the mutagen, the toxin significantly decreased the HU-induced aberration frequencies in NORs. The data obtained provide further evidence that α-amanitin is an effective modulator of aberration induction in NORs either by interfering with RNA synthesis or by modifying chromatin structure.