Literature DB >> 24253575

A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources.

A J Wilson1, P S Chourey.   

Abstract

A simplified method for the isolation of mitochondrial DNA (mtDNA) of several plant species from either coleoptile or tissue cultured cells is described. The procedure does not require gradient ultracentrifugation or organic solvent extractions (such as phenol, chloroform, ether, etc.). Protoplast isolation is not required for the release of organelles from cell suspension cultured cells. The entire procedure can be performed in a single day and employs differential low speed centrifugations for isolation of mitochondria and differential precipitations for the recovery of restrictable DNA.

Entities:  

Year:  1984        PMID： 24253575     DOI： 10.1007/BF00269301

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  9 in total

1.  Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize.

Authors:  D R Pring; C S Levings; W W Hu; D H Timothy
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

2.  A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA.

Authors:  F Nagy; G Lázár; L Menczel; P Maliga
Journal:  Theor Appl Genet       Date:  1983-09       Impact factor: 5.699

3.  Callus formation from protoplasts of a maize cell culture.

Authors:  P S Chourey; D B Zurawski
Journal:  Theor Appl Genet       Date:  1981-11       Impact factor: 5.699

4.  Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maize.

Authors:  J W McNay; P S Chourey; D R Pring
Journal:  Theor Appl Genet       Date:  1984-03       Impact factor: 5.699

5.  Plant protoplast fusion and growth of intergeneric hybrid cells.

Authors:  K N Kao; F Constabel; M R Michayluk; O L Gamborg
Journal:  Planta       Date:  1974-01       Impact factor: 4.116

6.  Classification of Normal and Male-Sterile Cytoplasms in Maize. II. Electrophoretic Analysis of DNA Species in Mitochondria.

Authors:  R J Kemble; R E Gunn; R B Flavell
Journal:  Genetics       Date:  1980-06       Impact factor: 4.562

7.  The effect of spermidine on endonuclease inhibition by agarose contaminants.

Authors:  J P Bouché
Journal:  Anal Biochem       Date:  1981-07-15       Impact factor: 3.365

8.  The mitochondrial genome is large and variable in a family of plants (cucurbitaceae).

Authors:  B L Ward; R S Anderson; A J Bendich
Journal:  Cell       Date:  1981-09       Impact factor: 41.582

9.  Mitochondrial DNA variation in maize plants regenerated during tissue culture selection.

Authors:  B G Gengenbach; J A Connelly; D R Pring; M F Conde
Journal:  Theor Appl Genet       Date:  1981-03       Impact factor: 5.699
  9 in total
  13 in total

1.  Identification of a nuclear-encoded sunflower mitochondrial tRNA-Leu (AAG).

Authors:  L R Ceci; M Dell'Orco; R Gallerani
Journal:  Plant Mol Biol       Date:  1992-08       Impact factor: 4.076

2.  AFLP studies on downy-mildew-resistant and downy-mildew-susceptible genotypes of opium poppy.

Authors:  Mukesh K Dubey; Ajit K Shasany; Om P Dhawan; Ashutosh K Shukla; Suman P S Khanuja
Journal:  J Genet       Date:  2010-04       Impact factor: 1.166

3.  Intraspecific diversity of sugar beet (Beta vulgaris) mitochondrial DNA.

Authors:  I K Komarnitsky; A M Samoylov; V V Red'ko; V G Peretyayko; Y Y Gleba
Journal:  Theor Appl Genet       Date:  1990-08       Impact factor: 5.699

4.  Mitochondrial genome variability in Sorghum cell culture protoclones.

Authors:  E J Kane; A J Wilson; P S Chourey
Journal:  Theor Appl Genet       Date:  1992-04       Impact factor: 5.699

5.  Electron microscopic investigation of mitochondrial DNA from Chenopodium album (L.).

Authors:  S Backert; R Lurz; T Börner
Journal:  Curr Genet       Date:  1996-04       Impact factor: 3.886

6.  Characterization of the S7 ribosomal protein gene in wheat mitochondria.

Authors:  D Zhuo; L Bonen
Journal:  Mol Gen Genet       Date:  1993-01

7.  Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun.

Authors:  Y I Ratushnyak; N N Cherep; A V Zavgorodnyaya; S A Latypov; I V Borozenko; R I Rachkovskaya; Y Y Gleba
Journal:  Mol Gen Genet       Date:  1993-01

8.  Investigation of plant organellar DNAs by pulsed-field gel electrophoresis.

Authors:  S Backert; P Dörfel; T Börner
Journal:  Curr Genet       Date:  1995-09       Impact factor: 3.886

9.  Properties of the linear N1 and N2 plasmid-like DNAs from mitochondria of cytoplasmic male-sterile Sorghum bicolor.

Authors:  C D Chase; D R Pring
Journal:  Plant Mol Biol       Date:  1986-01       Impact factor: 4.076

10.  Molecular analysis of the mitochondrial genome of Helianthus annuus in relation to cytoplasmic male sterility and phylogeny.

Authors:  D Crouzillat; P Leroy; A Perrault; G Ledoigt
Journal:  Theor Appl Genet       Date:  1987-10       Impact factor: 5.699
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