| Literature DB >> 24252336 |
Pierangelo Bellio1, Fabrizia Brisdelli1, Mariagrazia Perilli1, Alessia Sabatini1, Carlo Bottoni1, Bernardetta Segatore1, Domenico Setacci1, Gianfranco Amicosante1, Giuseppe Celenza2.
Abstract
The role of RecA protein in bacterial resistance to antibiotics makes this protein attractive from a pharmacological point of view. In this study we demonstrate that curcumin is able to inhibit the SOS response in Escherichia coli induced by levofloxacin. The blaTEM-1 gene has been placed under the control of the LexA-binding box and used as reporter gene. The expression of TEM-1 β-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8μg/ml, reduced dramatically the expression of the reporter gene. Moreover a simple microplate assay suitable for high-throughput screening has been developed.Entities:
Keywords: Bacterial SOS response; Curcumin; Levofloxacin
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Year: 2013 PMID： 24252336 DOI： 10.1016/j.phymed.2013.10.011
Source DB: PubMed Journal: Phytomedicine ISSN： 0944-7113 Impact factor: 5.340