Shixiang Guo1, Xuejun Xu1, Yichen Tang1, Chaobin Zhang1, Jian Li1, Yongsheng Ouyang1, Jingfang Ju2, Ping Bie3, Huaizhi Wang4. 1. Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. 2. Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA. Electronic address: jingfang.ju@stonybrookmedicine.edu. 3. Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: bieping@medmail.com.cn. 4. Institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: whuaizhi@gmail.com.
Abstract
PURPOSES: To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression. METHODS AND RESULTS: miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR-15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line. CONCLUSION: miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression.
PURPOSES: To investigate whether miR-15a inhibits cell proliferation and epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) via the down-regulation of B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) expression. METHODS AND RESULTS:miR-15a and Bmi-1 expressions in normal pancreatic tissue and PDAC tissue were measured. The relationship between miR-15a and Bmi-1 expression was analyzed. We found that miR-15a suppressed the expression of Bmi-1 and PDAC cell proliferation; E-cadherin expression was visibly up-regulated after silencing Bmi-1 by transfecting miR-15a into PDAC cell line. CONCLUSION:miR-15a inhibits cell proliferation and EMT in PDAC via the down-regulation of Bmi-1 expression.
Authors: Cho-Yi Chen; Ryan W Logan; Tianzhou Ma; David A Lewis; George C Tseng; Etienne Sibille; Colleen A McClung Journal: Proc Natl Acad Sci U S A Date: 2015-12-22 Impact factor: 11.205