INTRODUCTION: Human cord blood-derived stem cells are a rich source of stem cells as well as precursors. With regard to the researchers have focused on the therapeutic potential of stem cell in the neurological disease such as stroke, the aim of this study was the investiga-tion of the therapeutic effects of human cord blood-derived stem cells in cerebral ischemia on rat. METHODS: This study was carried out on young rats. Firstly, to create a laboratory model of ischemic stroke, carotid artery of animals was occluded for 30 minutes. Then, umbilical cord blood cells were isolated and labeled using bromodeoxyuridine and 2×10(5) cells were injected into the experimental group via the tail vein. Rats with hypoxic condi-tions were used as a sham group. A group of animals did not receive any injection or sur-geries were used as a control. RESULTS: Obtained results were evaluated based on behavioral responses and immunohistochemistry, with emphasis on areas of putamen and caudate nucleus in the control, sham and experimental groups. Our results indicated that behavioral recovery was observed in the experimental group compared to the either the sham or the control group. However, histological studies demonstrated a low percent of tissue injury in the experimental group in comparison with the sham group. CONCLUSION: Stem cell transplantation is beneficial for the brain tissue reparation after hypoxic ischemic cell death.
INTRODUCTION:Human cord blood-derived stem cells are a rich source of stem cells as well as precursors. With regard to the researchers have focused on the therapeutic potential of stem cell in the neurological disease such as stroke, the aim of this study was the investiga-tion of the therapeutic effects of human cord blood-derived stem cells in cerebral ischemia on rat. METHODS: This study was carried out on young rats. Firstly, to create a laboratory model of ischemic stroke, carotid artery of animals was occluded for 30 minutes. Then, umbilical cord blood cells were isolated and labeled using bromodeoxyuridine and 2×10(5) cells were injected into the experimental group via the tail vein. Rats with hypoxic condi-tions were used as a sham group. A group of animals did not receive any injection or sur-geries were used as a control. RESULTS: Obtained results were evaluated based on behavioral responses and immunohistochemistry, with emphasis on areas of putamen and caudate nucleus in the control, sham and experimental groups. Our results indicated that behavioral recovery was observed in the experimental group compared to the either the sham or the control group. However, histological studies demonstrated a low percent of tissue injury in the experimental group in comparison with the sham group. CONCLUSION: Stem cell transplantation is beneficial for the brain tissue reparation after hypoxic ischemic cell death.
Authors: Paul R Sanberg; Alison E Willing; Svitlana Garbuzova-Davis; Samuel Saporta; Guoqing Liu; Cyndy Davis Sanberg; Paula C Bickford; Stephen K Klasko; Nagwa S El-Badri Journal: Ann N Y Acad Sci Date: 2005-05 Impact factor: 5.691
Authors: Joost W Schouten; Carl T Fulp; Nicolas C Royo; Kathryn E Saatman; Deborah J Watson; Evan Y Snyder; John Q Trojanowski; Darwin J Prockop; Andrew I R Maas; Tracy K McIntosh Journal: J Neurotrauma Date: 2004-11 Impact factor: 5.269
Authors: A E Willing; J Lixian; M Milliken; S Poulos; T Zigova; S Song; C Hart; J Sanchez-Ramos; P R Sanberg Journal: J Neurosci Res Date: 2003-08-01 Impact factor: 4.164