Evaluation of Bactereuria and Antimicrobial Susceptibility among Hospitalized Patients With and Without Catheter in Kerman Province- Iran in 2011.

Nearly 15% to 25% of patients in general hospitals have a catheter at some time during their stay. Up to 97% of nosocomial urinary tract infections (UTIs) are related to catheter. The type of bacteremia is usually polymicrobial which makes the treatment more difficult. Previous studies showed an increase in mortality from bacteremia in these patients. The aim of the present study was to compare the prevalence of UTIs among patients with and without catheter, and to detect the type of bacteriuria and antibacterial resistance pattern. In this cross sectional study, samples were taken between Jan 2011 and July 2011. 678 hospitalized patients in different wards of Afzalipour hospital, Kerman- Iran, were enrolled in the study. E-test was applied to detect the pattern of resistance to gentamicin, nitrofurantoin, ciprofloxacin, ceftriaxon and co-trimoxazole. Results showed positive culture samples in 86% of female patients. Escherichia coli, Candida and Staphylococcus aureus were detected in 72, 20 and 7 percent of the positive cultures, respectively. 52.3% of detected E.coli was sensitive to gentamicin , 62% to ceftriaxone, 71.4% to ciprofloxacin, and 91.9% were sensitive to nitrofurantoin. Therefore, the most sensitive antibiotics in UTIs were ciprofloxacin and ceftriaxone. Unfortunately, the rate of antibacterial drug resistance was high in comparison with developed countries. Wise selection of antibiotics at hospitals and increasing the knowledge of patients to prevent self use of antibiotics can reduce antimicrobial resistance.

Introduction

The treatment of nosocomial infections is a costly procedure. Urinary tract infections (UTIs) account for 40-45% of hospital infections and cause spread of antibiotic resistant bacteria resources at the hospitals (1). Nearly 80% of hospital-acquired UTIs are associated with permanent catheter (2). Urinary catheter infections are common causes of nosocomial UTIs. Due to prevention of complete emptying of bladder, catheters can reduce the host’s natural defense and provide the environment for bacterial growth (3). Inserting a permanent catheter into the urethra in a hospitalized patient makes easy access to a sterilized location for pathogens (2). Up to 97% of nosocomial UTIs are associated with insertion of instruments, including catheter, into urinary tract. (4, 5), and annually account for up to 40% of nosocomial infections in the US hospitals (6- 8). UTIs often cause infection in long term care facilities (LTCFs) and most of them are catheter-associated (9, 10). Using catheter in the urinary tract is very prevalent in hospitals and LTCFs and seems to be increasing, at least in hospitals (11). Nearly 15% to 25% of patients in general hospitals have a catheter at some time during their stay (7, 12, and 13). Urethral catheterization manages nearly 5%to 10% of LTCFs residents (10, 13, 14), most of the time for bladder outlet block in men and urine incontinence of women (12, 15, 16). The period of catheterization is the most significant risk factor for development of catheter-associated bacteriuria (13, 17, 18). Nosocomial UTIs are often the most popular source of bacteremia in LTCFs, and the type of the bacteremia is usually polymicrobial in these patients (19, 20), which makes the treatment more difficult. Studies show that in these patients, mortality of bacteremia is three time more probable (21). Despite the limited and foreseeable spectrum of the causing agents in uncomplicated UTIs, a wide range of bactreria can cause nosocomial UTIs, and many are resistant to numerous antimicrobial drugs (22, 23). One of the most important problems in treating infectious diseases is lack of sufficient information from microorganism’s resistance pattern to antibiotics. The study conducted in 1997 in Bangladesh reported that the microbial resistance to co-trimoxazole was 43% (24). The other study in Qazvin-Iran in 1999 showed that Klebsiella pneumonia was the most common bacterial source related to catheter in genecology ward (25). The other study in 1997 showed the highest prevalence of microbial mass was related to the Escherichia coli (26). In 1998 in Tehran, the greatest resistance to usual antibiotics in urinary tract infections was co-trimoxazole (78.1%) (27). Since there is no sufficient data regarding antibiotic resistance to UTIs in Iran, we conducted this study in order to achieve practical patterns in treatment of infections by considering the amount and type of the bacteriuria and making decision to choose effective antibiotics in patients with and without catheter in our region. We hope that the information obtained in this research help us to find the way to reduce the costs of treatment and the period of hospitalization.

Experimental

In this cross-sectional study, samples were taken between Jan 2011 and July 2011 from all patients in different wards of Afzali-Pour general hospital, Kerman-Iran. 678 cases were enrolled in the study. According to having or not having the catheter, sampling methods were different. In patients without catheter, the middle part of the first morning urine sample were used and collected in disposable containers (midstream method). In patients who had urine catheter, samples were taken after catheter clamping, disinfecting outer part of the catheter and entering sterile syringes to the upper part of the catheter. Finally, urine samples were collected in 578 patients (85.63%) by midstream method and in 100 patients (14.37%) through the catheter. Samples were immediately transported to the laboratory and cultured on the blood agar environment (for differentiate types of Enterobacterias family). After 24 h incubation at 37°C, colonies were counted. Samples with colonies counted more than 105 were considered as positive (21). E-test strips (Biomerieux, Sweden) were used for antibiogram. This test uses a strip coated with a logarithmic gradient of an antimicrobial agent applied to an inoculated plate. After incubation, an ellipse of inhibition was formed. At the intersection of the ellipse with the strip, the MIC (minimum inhibitory concentration) was read from the interpretive scale and was converted to three sensitivity classes (sensitive, semi-sensitive, resistant) according to the breakpoints of Clinical and Laboratory Standards Institute (28). SPSS-16 was used for statistical analysis. Fisher’s exact test was used to determine the statistical differences for E. coli frequencies between patients with and without catheter.

Results

Patients were divided into two groups; with and without catheter. Among 578 patients without catheter, 53.8% were female, 59.4% were married and 42.3% had a history of other disease. In 100 patients who had catheter, 50% were female, 71% were married and 67% had a history of other disease. Out of 29 positive culture patients, 25 patients were female. Table 1 shows the frequency of UTI causing agents in patients. 649 out of 678 cases had negative cultures and only 4.3% of the total hospitalized patients had UTIs based on urine culture results.

Table 1

Frequency of infection causing agents among patients

		Negative culture	E.coli	Staphylococcus coagulase negative	Streptococ hemoliti	Candida	Klebsiella	Pseudomonas	Total
Without catheter	Number	560	14	2	1	1	0	0	578
	Percent	96.88%	2.43%	0.35%	0.17%	0.17%	0%	0%	100%
With catheter	Number	89	7	0	0	2	1	1	100
	Percent	89%	7%	0%	0%	2%	1%	1%	100%
Total	Number	649	21	2	1	3	1	1	678
	Percent	95.72%	3.1%	0.3%	0.15%	0.44%	0.15%	0.15%	100%
	p-value		0.024

Eleven percent of catheterized patients and 3.2% of patients without catheter had urine positive culture. Our study shows that E. coli was the leading cause of infection among our patients (72%). Frequency of E coli infection in catheter-associated patients was lower than patients without catheter (p = 0.024). Candida (10%) and Staphylococcus aureus (7%) were other important infection causing agents.

Tables 2 shows E.coli susceptibility to nitrofurantoin. 61.9% of the cases were sensitive and 9.5% of them were resistant to nitrofurantoin. Most of the patients who were sensitive to nitrofurantoin did not have catheter.

Table 2

Pattern of E.coli resistance to nitrofurantoin

		Sensitive			Semi-sensitive		Resistant		Total
		Number	Percent	Number	Percent	Number	Percent	Number	Percent
Without catheter			10	71.4%	3	%21.4	1	%7.2	14	100%
With catheter			3	42.8%	3	42.8%	1	14.4%	7	100%
Total			13	61.9%	6	28.6%	2	9.5%	21	100%
MIC (mcg/mL)			21		45		138

Table 3 shows that 52.3% of the cases were sensitive and 9.6% of them were resistant to gentamicin. Among the patients sensitive to gentamicin, 4 cases had catheter and 7 patients were without catheter. Table 4 shows that 62% of the patients were sensitive and 19% of them were resistant to ceftriaxon. Table 5 shows that totally, 71.4% of the patients were sensitive, and 14.3% were resistant to ciprofloxacin. More details have been shown in the tables.

Table 3

Pattern of E.coli resistance to gentamicin

		Sensitive		Semi-sensitive		Resistant		Total
		Number		Percent	Number	Percent	Number	Percent	Number	Percent
Without catheter	7		50%	5	35.7%	2	14.3%	14	100%
With catheter	4		57%	3	43%	0	0%	7	100%
Total	11		52.3%	8	38.1%	2	9.6%	21	100%
MIC (mcg/mL)	2			7		19

Table 4

Pattern of E.coli resistance to ceftriaxon

	Sensitive		Semi-sensitive		Resistant		Total
	Number	Percent	Number	Percent	Number	Percent	Number	Percent
Without catheter	9	64.3%	3	21.4%	2	14.3%	14	100%
With catheter	4	57.2%	1	12.3%	2	28.5%	7	100%
Total	13	62%	4	19%	4	19%	21	100%
MIC (mcg/mL)	5		27		76

Table 5

Pattern of E.coli resistance to ciprofloxacin

		Sensitive		Semi-sensitive		Resistant		Total
	Number		Percent	Number	Percent	Number	Percent	Number	Percent
Without catheter	10		71.4%	2	14.3%	2	14.3%	14	100%
With catheter	5		71.4%	1	14.3%	1	14.3%	7	100%
Total	15		71.4%	3	14.3%	3	14.3%	21	100%
MIC (mcg/mL)	0.8			1.4		5.6

Nonetheless, no significant difference was observed in the efficacy of the antibiotics in diabetic, renal and hypertensive patients (p > 0.05).

Discussion

In this study, for the first time, antimicrobial susceptibility was compared between hospitalized patients with or without urinary catheter. Most of the previous studies regarding antimicrobial resistance were performed using disk diffusion method that is old and almost non sensitive. We used E-test strip that is more sensitive and could show MIC for different antimicrobial agents. In this study, the frequency of catheterization between women and men was the same, but the rate of catheterization in married patients was significantly more than single patients. There was no significant difference between the gender of the patients who were involved the study. In catheterized patients, 50% and in patients without catheter 53% were female. As the previous study showed (29), women (86%) are more susceptible to UTIs in comparison to men in our study.

Surprisingly, only 4.3% of the hospitalized patients were culture positive. possibly because most of our patients took antibiotic before admission to the hospital. Almost all of the Antibiotics (oral or parenteral) can bought easily and without a prescription from pharmacies around the city. These drugs are inexpensive, and most of the patients prefer to do self-medication once they got symptoms of infection.

This study showed that the most sensitive antibiotic for E.coli in patients with underlying cardiovascular disease and also in patients with diabetes was gentamicin, however, the antibiotic of choice in patients with renal disease was ciprofloxacin. In this study, the most common cause of urinary tract infections was E.coli, and ciprofloxacin was also a good choice because of the low microbial resistance compared with other evaluated antibiotics.

In the study conducted in Brazil, E.coli (26%), Pseudomonas aeruginosa (15%) and Klebsiella pneumonia (11%) were the main causes of UTIs. In that research, the rate of E.coli sensitivity was 93% to ceftriaxone and 78% to ciprofloxacin.(30). However, in our study E.coli was detected in 72% of the patients, and the sensitivity to ciprofloxacin (71.4%) was interestingly higher than ceftriaxone (62%). It may be related to misuse of ceftriaxon in our community which is because of patients’ desire for injectable medicine, emphasis on prescribing injectable drugs, and cultural problem and misleading information about the efficacy of injectable medicine in comparison with oral medications. Researches in two hospitals in Kuwait during one year have shown that the most common microbial masses were E.coli (47%), Candida (10%) and Klebsiella pneumoniae (9.6%) (31). A research in Medical College Hospital in India showed that the most common bacterial causes of UTIs were Staphylococcus aureus ( 27%) and E.coli (21%) [26], but our results were different. Previous study conducted in 2000 in Kerman showed that E.coli was the microbial cause of UTIs in 63% of patients. E.coli susceptibility was around 69% to nitrofurantoin, 92% to ciprofloxacin, 37% to gentamicin and 38% to co-trimoxazole (32). Whereas in our study and after 11 years, E.coli sensitivity to gentamicin increased by 52.3%. It may be because of introducing ceftriaxon to market and over-prescribing of the drug. It seems that gentamicin was replaced with ceftriaxon here in patients who need injectable medicine for UTIs. However, ciprofloxacin sensitivity had decreased from 92% to 71.4%. In a research performed in the gynecological department of Qazvin-Iran University hospital, urine samples taken from146 catheter-associated patients showed the following microbial pattern: Klebsiella pneumoniae 60%, E.coli 25%, Proteus mirabilis 5%, Pseudomonas aeruginosa 2.5% (25). Studies in Bangladesh in 1997 showed that the microorganism sensitivity to ciprofloxacin was74% and this drug was more active than other antibiotics against Gram-negative bacteria (24) which is consistent with our results In the other study in the ICU of Afzalipour hospital, the most common identified microorganisms were Klebsiella (90.6%), Acinetobacter (28.1%) and Pseudomonas (21.9%) (33). It was concluded that ICU can have different microbial frequency from general wards. As regarded in this research, the most sensitive antibiotics in UTIs are ciprofloxacin and ceftriaxone. They are broad-spectrum antibiotics, and irrational use of them leads to an increase in the rate of microbial resistance not only in UTIs, but also in all the infectious disease. However, irrational use of ceftriaxon in our country resulted in 60 patient’s death. Hence, practitioner must be aware of the slow infusion and incompatibility with calcium ions (34). Our results showed that catheter associated patients were more resistant to nitrofurantoin and ceftriaxon. Drug and therapeutic committees (DTC) can help medical staff to choose antibiotics wisely in each field based on the guidelines or protocols. Standard hospital pharmacy unit and hospital or clinical pharmacists can prevent the development of antibiotic resistance by preparing injectable medicine under aseptic condition, and helping physicians choose the proper antibiotics based on patients sample culture results and avoiding prolonged catheterization.