| Literature DB >> 2425001 |
L M Pendy, S J Arra, L M Anselmino, L L Thomas.
Abstract
A flow cytometric procedure is described for the isolation of human basophils. The basophils are isolated by cell sorting after passive sensitization with mouse FITC-IgE anti-DNP, and the isolated basophils exhibit characteristic histamine release in response to incubation with DNP21-human serum albumin conjugates. Using this method, 1-4 X 10(5) basophils can be isolated at greater than or equal to 98% purity from approximately 30 ml whole blood in a 2 h sorting procedure.Entities:
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Year: 1986 PMID： 2425001 DOI： 10.1016/0022-1759(86)90102-x
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303