Quantitative and temporal analysis of effects of twospotted spider mite (acari: Tetranychidae) female sex pheromone on male guarding behavior.

Responses of male twospotted spider mite,Tetranychus urticae (Koch), to female sex pheromone were described by a glass slide bioassay and computerized pathway digitizer. Pheromone was extracted from quiescent deutonymphs and fractionated by HPLC, and responses of guarding males to each fraction, all fractions combined, unfractionated extract, and a hexane control were bioassayed for 6 min. Mean angular velocities, linear velocities, percent time stationary, and distance from the 3-mm-diameter treatment circle were calculated for each mite at 20-sec intervals and these behavioral parameters regressed on time. Analysis of variance of regression-equation intercepts showed that no differences in initial male angular velocities were observed among treatments, but initial linear velocities were greater in response to all fractions combined and to extract than to individual fractions, and greater in response to individual fractions than to the control. Angular velocities decreased and linear velocities increased more rapidly in response to individual fractions than to all fractions combined and extract, while males turned preferentially in one direction and were stationary (no displacement) more often in response to all-fraction combinations than to individual fractions and the control. Significant differences were observed among the parameter values elicited by individual fractions, suggesting that active fractions differed qualitatively. The described changes in movement parameters show that maleT. urticae response to presence of pheromone occurs almost immediately, and that multiple pheromonal components are necessary to elicit maximum male response. The overall effect of pheromonal components was to decrease the rate at which males moved away from the treatment cycle, thereby causing male arrestment.