Biologically active secondary metabolites of barley. III. A method for identification and quantification of hordenine and gramine in barley by high-performance liquid chromatography.

A method was devised for the extraction and quantification of hordenine and gramine from barley (Hordeum vulgare) tissue using HPLC techniques. Quantification was by peak area, the relationship between peak area and concentration of authentic standards being linear for both hordenine and gramine. Significant differences in the ability of three lines of barley to produce hordenine and gramine were detected using this method.