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Literature DB >> 24248456

A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis.

Abstract

A fast and simple extraction procedure of plant tissue for two-dimensional gel electrophoresis is presented. The procedure is especially useful for the extraction of plant cell suspension cultures, callus and other plant tissues having a high content of phenol oxidases, polysaccharides, polynucleotides, terpenoids and other substances interfering with isoelectric focusing. Due to the speed of the extraction procedure (about 20 min), large numbers of samples containing only milligram amounts of tissue can be easily processed. The simplicity of the method makes it particularly suitable for the extraction of radiolabeled tissues ((35)S, (32)P). This method is perfectly compatible with silver staining, autoradiography and Western blotting analysis.

Entities: Chemical

Year: 1987 PMID： 24248456 DOI： 10.1007/BF00269745

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

11 in total

1. A new procedure for the isolation and fractionation of histones.

Authors: D R. van der Westhuyzen; C von Holt
Journal: FEBS Lett Date: 1971-05-20 Impact factor: 4.124

2. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

3. Physical studies on the H3/H4 histone tetramer.

Authors: T Moss; P D Cary; C Crane-Robinson; E M Bradbury
Journal: Biochemistry Date: 1976-06-01 Impact factor: 3.162

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Two-dimensional gel electrophoresis of membrane proteins.

Authors: G F Ames; K Nikaido
Journal: Biochemistry Date: 1976-02-10 Impact factor: 3.162

8. Artifacts associated with 2-mercaptoethanol upon high resolution two-dimensional electrophoresis.

Authors: T Marshall; K M Williams
Journal: Anal Biochem Date: 1984-06 Impact factor: 3.365

9. Resolution of basic cellular proteins including histone variants by two-dimensional gel electrophoresis: evaluation of lysine to arginine ratios and phosphorylation.

Authors: M M Sanders; V E Groppi; E T Browning
Journal: Anal Biochem Date: 1980-03-15 Impact factor: 3.365

6. Glutamine synthetases of green and etiolated leaves ofSinapis alba : Evidence of the identity of the respective enzyme proteins.

Authors: M Höpfner; G Ochs; A Wild
Journal: Planta Date: 1990-05 Impact factor: 4.116

6 in total

A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis.

1. A new procedure for the isolation and fractionation of histones.

2. High resolution two-dimensional electrophoresis of proteins.

3. Physical studies on the H3/H4 histone tetramer.

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Two-dimensional gel electrophoresis of membrane proteins.

8. Artifacts associated with 2-mercaptoethanol upon high resolution two-dimensional electrophoresis.

9. Resolution of basic cellular proteins including histone variants by two-dimensional gel electrophoresis: evaluation of lysine to arginine ratios and phosphorylation.

10. Characterization of the ribosomal proteins from mosquito (Aedes albopictus) cells.

1. Relation of the developmental stage of zygotic embryos of yellow-poplar to their somatic embryogenic potential.

2. Changes in ovule protein profiles associated with somatic embryogenesis in Cercis canadensis (redbud).

3. Characterization of two genes encoding small heat-shock proteins in Arabidopsis thaliana.

4. Arabidopsis thaliana expressing the cauliflower mosaic virus ORF VI transgene has a late flowering phenotype.

5. Messenger RNA induction in cellular salt tolerance of Alfalfa (Medicago sativa).

6. Glutamine synthetases of green and etiolated leaves ofSinapis alba : Evidence of the identity of the respective enzyme proteins.