Literature DB >> 24248454

Nitro-blue tetrazolium: A specific stain for photosynthetic activity in protoplasts.

D Robertson1, E D Earle.   

Abstract

A qualitative assay for the detection of photosynthetic activity in protoplasts is described. Leaf protoplasts from atrazine resistant and susceptible biotypes of Brassica napus suspended in medium with 0.01% nitro-blue tetrazolium and 10 to 100 µM atrazine showed clear differences in staining. Staining was detectable with 0.001% fluorescein-labelled tetrazolium which, unlike nitro-blue tetrazolium, did not cause collapse of the protoplasts.

Entities:  

Year:  1987        PMID： 24248454     DOI： 10.1007/BF00269743

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  5 in total

1.  Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli.

Authors:  D Robertson; E D Earle
Journal:  Plant Cell Rep       Date:  1986-02       Impact factor: 4.570

2.  Cytochemical and cytofluorometric evidence for guard cell photosystems.

Authors:  K C Vaughn; W H Outlaw
Journal:  Plant Physiol       Date:  1983-02       Impact factor: 8.340

3.  Uniparental inheritance of a chloroplast photosystem II polypeptide controlling herbicide binding.

Authors:  S Darr; V Souza Machado; C J Arntzen
Journal:  Biochim Biophys Acta       Date:  1981-01-14

4.  On the use of Avena protoplasts to study chloroplast development.

Authors:  R Hampp; H Ziegler
Journal:  Planta       Date:  1980-02       Impact factor: 4.116

5.  Photosynthesis of leaf cell protoplasts and permeability of the plasmalemma to some solutes.

Authors:  G Kaiser; U Heber
Journal:  Planta       Date:  1983-04       Impact factor: 4.116
  5 in total
  4 in total

1.  Atrazine-resistant cytoplasmic male-sterile-nigra broccoli obtained by protoplast fusion between cytoplasmic male-sterile Brassica oleracea and atrazine-resistant Brassica campestris.

Authors:  M C Christey; C A Makaroff; E D Earle
Journal:  Theor Appl Genet       Date:  1991-12       Impact factor: 5.699

2.  Atrazine-resistant cauliflower obtained by somatic hybridization between Brassica oleracea and ATR-B. napus.

Authors:  P S Jourdan; E D Earle; M A Mutschler
Journal:  Theor Appl Genet       Date:  1989-08       Impact factor: 5.699

3.  Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris.

Authors:  P S Jourdan; E D Earle; M A Mutschler
Journal:  Theor Appl Genet       Date:  1989-09       Impact factor: 5.699

4.  Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris.

Authors:  D Robertson; J D Palmer; E D Earle; M A Mutschler
Journal:  Theor Appl Genet       Date:  1987-07       Impact factor: 5.699
  4 in total

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