Literature DB >> 24248209

Behaviour of chromosomes in potato leaf tissue cultured in vitro as studied by BrdC-Giemsa labelling.

L P Pijnacker1, K Walch, M A Ferwerda.   

Abstract

Cells of leaf explants of a monohaploid potato (Solanum tuberosum) were stimulated to mitosis on a medium with 5-bromodeoxycytidine during a period of 7 days. The cells cycled with mono- or diplochromosomes which showed differential staining of the sister chromatids and sister chromatid exchanges by the fluorescent plus Giemsa technique after two rounds of BrdC incorporation. Through the staining pattern the course of the first three cell cycles could be traced and the duration of the cycles estimated. Polyploidisation was enhanced by selective stimulation of polyploid cells and by endoreduplication of G2-phase cells. The percentage of polyploid mitoses increased from 10 to 70.

Entities:  

Year:  1986        PMID： 24248209     DOI： 10.1007/BF00266554

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  13 in total

1.  THE VALIDITY OF AUXIN-INDUCED DIVISIONS IN PLANTS AS EVIDENCE OF ENDOPOLYPLOIDY.

Authors:  C R PARTANEN
Journal:  Exp Cell Res       Date:  1963-09       Impact factor: 3.905

2.  DNA synthesis, cell division and specific cytodifferentiation in cultured pea root cortical explants.

Authors:  R Phillips; J G Torrey
Journal:  Dev Biol       Date:  1973-04       Impact factor: 3.582

3.  Insights on diplochromosome structure and behaviour.

Authors:  V J Goyanes; J B Schvartzman
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

Review 4.  Sister chromatid exchange formation.

Authors:  S A Latt
Journal:  Annu Rev Genet       Date:  1981       Impact factor: 16.830

Review 5.  Sister chromatid exchange.

Authors:  S Wolff
Journal:  Annu Rev Genet       Date:  1977       Impact factor: 16.830

6.  Timing of endoreduplication induced by colcemid or radiation in BUdR-labeled human lymphocytes.

Authors:  K E Weber; S F Hoegerman
Journal:  Exp Cell Res       Date:  1980-07       Impact factor: 3.905

7.  Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining.

Authors:  K Goto; T Akematsu; H Shimazu; T Sugiyama
Journal:  Chromosoma       Date:  1975-12-10       Impact factor: 4.316

8.  Studies on endoreduplication. II. Spontaneous occurrence and cellular kinetics of endoreduplication in PHA-stimulated tonsillar lymphocytes.

Authors:  H Takanari; K Izutsu
Journal:  Cytogenet Cell Genet       Date:  1981

9.  [Sister chromatid exchanges in bromine incorporation into DNA cytosine nucleotides. III. 5-bromodeoxycytidine as a DNA thymine precursor. The characteristics of the exchanges].

Authors:  T L Baĭramian; A F Zakharov
Journal:  Tsitologiia       Date:  1979-04

10.  Comparisons of in vivo BrdU labeling methods and spontaneous sister chromatid exchange frequencies in regenerating murine liver and bone marrow cells.

Authors:  M K Conner; S S Boggs; J H Turner
Journal:  Chromosoma       Date:  1978-09-11       Impact factor: 4.316
View more
  9 in total

1.  Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants.

Authors:  J Puonti-Kaerlas; T Eriksson; P Engström
Journal:  Theor Appl Genet       Date:  1992-07       Impact factor: 5.699

2.  Sister chromatid exchanges in cultured immature embryos of wheat species and regenerants.

Authors:  L P Pijnacker; M A Ferwerda
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

3.  Nucleolar organizer function in developing potato calli.

Authors:  L P Pijnacker; M A Ferwerda
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

4.  Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by "gamma-fusion".

Authors:  Y Y Gleba; S Hinnisdaels; V A Sidorov; V A Kaleda; A S Parokonny; N V Boryshuk; N N Cherep; I Negrutiu; M Jacobs
Journal:  Theor Appl Genet       Date:  1988-11       Impact factor: 5.699

5.  In situ hybridization to somatic metaphase chromosomes of potato.

Authors:  R G Visser; R Hoekstra; F R van der Leij; L P Pijnacker; B Witholt; W J Feenstra
Journal:  Theor Appl Genet       Date:  1988-09       Impact factor: 5.699

6.  Flow cytometric and karyological analysis of polysomaty and polyploidization during callus formation from leaf segments of various potato genotypes.

Authors:  L P Pijnacker; K Sree Ramulu; P Dijkhuis; M A Ferwerda
Journal:  Theor Appl Genet       Date:  1989-01       Impact factor: 5.699

7.  Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry.

Authors:  B A Uijtewaal
Journal:  Plant Cell Rep       Date:  1987-06       Impact factor: 4.570

8.  Karyotype variation in aminoethylcysteine resistant cell and callus cultures and regenerated plants of a dihaploid potato (Solanum tuberosum).

Authors:  L P Pijnacker; M A Ferwerda
Journal:  Plant Cell Rep       Date:  1987-10       Impact factor: 4.570

9.  Chromosome variation in dividing protoplasts and cell suspensions of wheat.

Authors:  A Karp; Q S Wu; S H Steele; M G Jones
Journal:  Theor Appl Genet       Date:  1987-05       Impact factor: 5.699
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.