Literature DB >> 2424336

Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

E H Ball.   

Abstract

A simple method for the extraction of Coomassie brilliant blue R from stained protein bands excised from polyacrylamide gels is described. Spectrophotometric measurement of the eluted dye forms the basis of a sensitive assay to quantitate proteins in gels in the range 0.5-10 micrograms. The method requires no unusual equipment and is suitable for measurement of multiple samples. The polypeptide is not extracted and remains available for further analysis. The technique has been applied to three proteins and gels of various acrylamide percentages.

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Year:  1986        PMID： 2424336     DOI： 10.1016/0003-2697(86)90218-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Conformational changes produced by ATP binding to the plasma membrane calcium pump.

Authors:  Irene C Mangialavori; Mariela S Ferreira-Gomes; Nicolás A Saffioti; Rodolfo M González-Lebrero; Rolando C Rossi; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2013-09-11       Impact factor: 5.157

2.  Antibodies to synthetic peptides from the tubulin regulatory domain interact with tubulin and microtubules.

Authors:  J C Vera; C I Rivas; R B Maccioni
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

3.  Localization of the membrane-associated region of vesicular stomatitis virus M protein at the N terminus, using the hydrophobic, photoreactive probe 125I-TID.

Authors:  J Lenard; R Vanderoef
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

4.  High mobility group proteins cHMG1a, cHMG1b, and cHMGI are distinctly distributed in chromosomes and differentially expressed during ecdysone dependent cell differentiation.

Authors:  S Ghidelli; P Claus; G Thies; J R Wiśniewski
Journal:  Chromosoma       Date:  1997-04       Impact factor: 4.316

5.  Autoinhibition mechanism of the plasma membrane calcium pump isoforms 2 and 4 studied through lipid-protein interaction.

Authors:  Irene C Mangialavori; Gerardo Corradi; Débora E Rinaldi; María Candelaria de la Fuente; Hugo P Adamo; Juan Pablo F C Rossi
Journal:  Biochem J       Date:  2012-04-01       Impact factor: 3.857

6.  Ethrel-stimulated prolongation of latex flow in the rubber tree (Hevea brasiliensis Muell. Arg.): an Hev b 7-like protein acts as a universal antagonist of rubber particle aggregating factors from lutoids and C-serum.

Authors:  Min-Jing Shi; Fu-Ge Cai; Wei-Min Tian
Journal:  J Biochem       Date:  2015-09-17       Impact factor: 3.387

7.  A new conformation in sarcoplasmic reticulum calcium pump and plasma membrane Ca2+ pumps revealed by a photoactivatable phospholipidic probe.

Authors:  Irene Mangialavori; Ana María Villamil Giraldo; Cristina Marino Buslje; Mariela Ferreira Gomes; Ariel J Caride; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2008-12-12       Impact factor: 5.157

8.  Differential effects of G- and F-actin on the plasma membrane calcium pump activity.

Authors:  Laura Vanagas; María Candelaria de La Fuente; Marianela Dalghi; Mariela Ferreira-Gomes; Rolando C Rossi; Emanuel E Strehler; Irene C Mangialavori; Juan P F C Rossi
Journal:  Cell Biochem Biophys       Date:  2013-05       Impact factor: 2.194

9.  Anti-idiotypic antibodies that react with microtubule-associated proteins are present in the sera of rabbits immunized with synthetic peptides from tubulin's regulatory domain.

Authors:  C I Rivas; J C Vera; R B Maccioni
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

10.  Determination of the dissociation constants for Ca2+ and calmodulin from the plasma membrane Ca2+ pump by a lipid probe that senses membrane domain changes.

Authors:  Irene Mangialavori; Mariela Ferreira-Gomes; María F Pignataro; Emanuel E Strehler; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2009-11-05       Impact factor: 5.157
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