Literature DB >> 24243209

A protocol for high-throughput Agrobacterium-mediated barley transformation.

Wendy A Harwood1.   

Abstract

Agrobacterium-mediated transformation of barley is a valuable tool for determining gene function by over-expression of a gene of interest or by RNAi-based gene silencing. The method is based on the inoculation of immature embryos with Agrobacterium and uses a hygromycin resistance gene to allow selection of transgenic plants. The protocol described leads to average transformation efficiencies of 25 % meaning that large numbers of fertile transgenic plants can be produced.

Entities:  

Mesh：

Year:  2014        PMID： 24243209     DOI： 10.1007/978-1-62703-715-0_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  A Trypsin Family Protein Gene Controls Tillering and Leaf Shape in Barley.

Authors:  Lingzhen Ye; Yin Wang; Lizhi Long; Hao Luo; Qiufang Shen; Sue Broughton; Dianxing Wu; Xiaoli Shu; Fei Dai; Chengdao Li; Guoping Zhang
Journal:  Plant Physiol       Date:  2019-08-19       Impact factor: 8.340

2.  The HKT Transporter HvHKT1;5 Negatively Regulates Salt Tolerance.

Authors:  Lu Huang; Liuhui Kuang; Liyuan Wu; Qiufang Shen; Yong Han; Lixi Jiang; Dezhi Wu; Guoping Zhang
Journal:  Plant Physiol       Date:  2019-11-05       Impact factor: 8.340

3.  Expression of the high molecular weight glutenin 1Ay gene from Triticum urartu in barley.

Authors:  Qiang Yang; Siyu Li; Xiaoyu Li; Jian Ma; Jirui Wang; Pengfei Qi; Guoyue Chen; Zhien Pu; Wei Li; Wendy Harwood; Zhongyi Li; Bao-Long Liu; Xiujin Lan; Mei Deng; Zhenxiang Lu; Yuming Wei; Youliang Zheng; Qiantao Jiang
Journal:  Transgenic Res       Date:  2019-02-27       Impact factor: 2.788

4.  Functional dissection of HGGT and HPT in barley vitamin E biosynthesis via CRISPR/Cas9-enabled genome editing.

Authors:  Zhanghui Zeng; Ning Han; Cuicui Liu; B Buerte; Chenlu Zhou; Jianshu Chen; Mengyao Wang; Yuhong Zhang; Yawei Tang; Muyuan Zhu; Junhui Wang; Yinong Yang; Hongwu Bian
Journal:  Ann Bot       Date:  2020-10-06       Impact factor: 4.357

5.  Vacuolar H+-pyrophosphatase HVP10 enhances salt tolerance via promoting Na+ translocation into root vacuoles.

Authors:  Liangbo Fu; Dezhi Wu; Xincheng Zhang; Yunfeng Xu; Liuhui Kuang; Shengguan Cai; Guoping Zhang; Qiufang Shen
Journal:  Plant Physiol       Date:  2022-02-04       Impact factor: 8.340

6.  Engineered plant control of associative nitrogen fixation.

Authors:  Timothy L Haskett; Ponraj Paramasivan; Marta D Mendes; Patrick Green; Barney A Geddes; Hayley E Knights; Beatriz Jorrin; Min-Hyung Ryu; Paul Brett; Christopher A Voigt; Giles E D Oldroyd; Philip S Poole
Journal:  Proc Natl Acad Sci U S A       Date:  2022-04-11       Impact factor: 12.779

Review 7.  Progress of cereal transformation technology mediated by Agrobacterium tumefaciens.

Authors:  Yukoh Hiei; Yuji Ishida; Toshihiko Komari
Journal:  Front Plant Sci       Date:  2014-11-07       Impact factor: 5.753

8.  Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria.

Authors:  Barney A Geddes; Ponraj Paramasivan; Amelie Joffrin; Amber L Thompson; Kirsten Christensen; Beatriz Jorrin; Paul Brett; Stuart J Conway; Giles E D Oldroyd; Philip S Poole
Journal:  Nat Commun       Date:  2019-07-31       Impact factor: 14.919

9.  A highly mutagenised barley (cv. Golden Promise) TILLING population coupled with strategies for screening-by-sequencing.

Authors:  Miriam Schreiber; Abdellah Barakate; Nicola Uzrek; Malcolm Macaulay; Adeline Sourdille; Jenny Morris; Pete E Hedley; Luke Ramsay; Robbie Waugh
Journal:  Plant Methods       Date:  2019-08-24       Impact factor: 4.993

10.  Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9.

Authors:  Rhian M Howells; Melanie Craze; Sarah Bowden; Emma J Wallington
Journal:  BMC Plant Biol       Date:  2018-10-03       Impact factor: 4.215
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