Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons.

Literature DB >> 24241425

Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons.

Abstract

A method is described for separating mitochondria from microbodies in cotyledon preparations of Pisum sativum L. cv. Alaska. Pure and intact mitochondria were obtained on a continuous: discontinuous sucrose density gradient as shown by marke-enzyme assay and electron microscopy. Manipulation of sucrose-gradient construction to widen the distance between organelles provided a quick method for the separation of the mitochondria from the microbodies. The shorter time of exposure of mitochondria to centrifugation and osmotic stress produces mitochondria free of contamination.

Entities: Chemical Species

Year: 1985 PMID： 24241425 DOI： 10.1007/BF00397341

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

9 in total

1. Studies on the regulation of chloroplast NADP-linked glyceraldehyde-3-phosphate dehydrogenase.

Authors: R A Wolosiuk; B B Buchanan
Journal: J Biol Chem Date: 1976-10-25 Impact factor: 5.157

2. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors: D I Arnon
Journal: Plant Physiol Date: 1949-01 Impact factor: 8.340

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

Authors: M A Markwell; S M Haas; L L Bieber; N E Tolbert
Journal: Anal Biochem Date: 1978-06-15 Impact factor: 3.365

5. Biochemical and structural changes in mitochondria and other cellular components of pea cotyledons during germination.

Authors: T Solomos; S S Malhotra; S Prasad; S K Malhotra; M Spencer
Journal: Can J Biochem Date: 1972-07

6. Preparation of intaintact plant mitochondria.

Authors: R Douce; E L Christensen; W D Bonner
Journal: Biochim Biophys Acta Date: 1972-08-17

7. Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis.

Authors: G von Jagow; M Klingenberg
Journal: Eur J Biochem Date: 1970-02

8. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.

Authors: E RACKER
Journal: Biochim Biophys Acta Date: 1950-01

9. The synthesis of palmitoylcarnitine by etio-chloroplasts of greening barley leaves.

Authors: D R Thomas; M N Jalil; R J Cooke; B C Yong; A Ariffin; P H McNeil; C Wood
Journal: Planta Date: 1982-03 Impact factor: 4.116

9 in total

8 in total

6. The two β-oxidation sites in pea cotyledons : Carnitine palmitoyltransferase: location and function in pea mitochondria.

Authors: D R Thomas; C Wood
Journal: Planta Date: 1986-06 Impact factor: 4.116

7. β-Oxidation enzymes in the mitochondria of Arum and oilseed rape.

Authors: C Masterson; C Wood; D R Thomas
Journal: Planta Date: 1990-08 Impact factor: 4.116

8. Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis.

Authors: Wenyun Shen; Yangdou Wei; Melanie Dauk; Yifang Tan; David C Taylor; Gopalan Selvaraj; Jitao Zou
Journal: Plant Cell Date: 2006-01-13 Impact factor: 11.277