Literature DB >> 24241413

Partial purification and characterization of dihydrobenzophenanthridine oxidase from Eschscholtzia californica cell suspension cultures.

H M Schumacher1, M H Zenk.   

Abstract

The observation that upon elicitation cell suspension cultures of Eschscholtzia california showed a decrease of dihydromacarpine with a concomittant increase of macarpine led to the discovery of a novel enzyme which catalyzes the oxidation of dihydrobenzophenanthridines in the presence of oxygen. The enzyme was enriched approx. 70-fold. It has a pH-optimum of 7.0, an isoelectric point at pH 8.8, molecular weight of 56 kD and shows a high degree of substrate specificity. The enzyme obviously catalyzes the terminal step in the formation of benzophenanthridine alkaloids containing methylene dioxy substitutions in rings A and D.

Entities:  

Year:  1988        PMID： 24241413     DOI： 10.1007/BF00272975

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  2 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Elicitation of benzophenanthridine alkaloid synthesis in Eschscholtzia cell cultures.

Authors:  H M Schumacher; H Gundlach; F Fiedler; M H Zenk
Journal:  Plant Cell Rep       Date:  1987-12       Impact factor: 4.570
  2 in total
  7 in total

1.  Integration of deep transcript and targeted metabolite profiles for eight cultivars of opium poppy.

Authors:  Isabel Desgagné-Penix; Scott C Farrow; Dustin Cram; Jacek Nowak; Peter J Facchini
Journal:  Plant Mol Biol       Date:  2012-04-24       Impact factor: 4.076

2.  Gene transcript and metabolite profiling of elicitor-induced opium poppy cell cultures reveals the coordinate regulation of primary and secondary metabolism.

Authors:  Katherine G Zulak; Anthony Cornish; Timothy E Daskalchuk; Michael K Deyholos; Dayan B Goodenowe; Paul M K Gordon; Darren Klassen; Lawrence E Pelcher; Christoph W Sensen; Peter J Facchini
Journal:  Planta       Date:  2006-11-01       Impact factor: 4.116

3.  Characterization of a flavoprotein oxidase from opium poppy catalyzing the final steps in sanguinarine and papaverine biosynthesis.

Authors:  Jillian M Hagel; Guillaume A W Beaudoin; Elena Fossati; Andrew Ekins; Vincent J J Martin; Peter J Facchini
Journal:  J Biol Chem       Date:  2012-11-01       Impact factor: 5.157

4.  Semi-continuous production of sanguinarine and dihydrosanguinarine by Papaver somniferum L. cell suspension cultures treated with fungal homogenate.

Authors:  R T Tyler; U Eilert; C O Rijnders; I A Roewer; W G Kurz
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570

5.  Integration of deep transcriptome and proteome analyses reveals the components of alkaloid metabolism in opium poppy cell cultures.

Authors:  Isabel Desgagné-Penix; Morgan F Khan; David C Schriemer; Dustin Cram; Jacek Nowak; Peter J Facchini
Journal:  BMC Plant Biol       Date:  2010-11-18       Impact factor: 4.215

6.  Influence of different elicitors on BIA production in Macleaya cordata.

Authors:  Peng Huang; Liqiong Xia; Li Zhou; Wei Liu; Peng Wang; Zhixing Qing; Jianguo Zeng
Journal:  Sci Rep       Date:  2021-01-12       Impact factor: 4.379

7.  Wound induced tanscriptional regulation of benzylisoquinoline pathway and characterization of wound inducible PsWRKY transcription factor from Papaver somniferum.

Authors:  Sonal Mishra; Vineeta Triptahi; Seema Singh; Ujjal J Phukan; M M Gupta; Karuna Shanker; Rakesh Kumar Shukla
Journal:  PLoS One       Date:  2013-01-30       Impact factor: 3.240
  7 in total

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