Effect of cytokinin on morphological changes of suspension cultured cells of the moss, Barbula unguiculata.

Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited by the addition of 5 μM benzyladenine or 6-furfurylaminopurine but is not affected by the addition of 5 μM 2,4-dichlorophenoxyacetic acid or naphthalene acetic acid.