Long-term effect of electroporation on enhancement of growth and plant regeneration of colt cherry (Prunus avium × pseudocerasus) protoplasts.

Electric pulses applied to Colt cherry protoplasts enhanced the long-term growth and plant regeneration of protoplast-derived tissues. Protoplasts isolated from long-term cultured tissues derived from electroporated protoplasts retained the ability to enter division in culture earlier and with a higher frequency of plant regeneration than untreated cell suspension protoplasts.