BACKGROUND: Adipose tissue-derived stem cells (ADSCs), which are widely known as multipotent progenitor cells, release several cytokines that support cell survival and repair. The aim of this study was to investigate whether ADSC-secreted molecules could induce a trophic effect in pancreatic islet culture conditions in vitro. MATERIALS AND METHODS: We cocultured porcine islet cells with ADSCs using a transwell system for 48 h and evaluated the viability of islet cells. We also determined the concentration levels of cytokines and insulin in the supernatant of the culture medium. We used anti-vascular endothelial growth factor (VEGF) and anti-interleukin (IL)-6 receptor antibodies to investigate the effect of VEGF and IL-6 on islet cells. RESULTS: ADSCs improved the viability of islet cells in the absence of cell-cell contact (P < 0.05). VEGF and IL-6 levels in the culture medium increased when islet cells were cocultured with ADSCs (P < 0.05). Furthermore, inhibition of VEGF decreased the viability of islet cells (P < 0.05); however, inhibition of IL-6 did not affect islet cell viability. CONCLUSIONS: These results suggested that trophic factors, particularly VEGF, secreted by human ADSCs enhanced the survival and function of porcine islet cells.
BACKGROUND: Adipose tissue-derived stem cells (ADSCs), which are widely known as multipotent progenitor cells, release several cytokines that support cell survival and repair. The aim of this study was to investigate whether ADSC-secreted molecules could induce a trophic effect in pancreatic islet culture conditions in vitro. MATERIALS AND METHODS: We cocultured porcine islet cells with ADSCs using a transwell system for 48 h and evaluated the viability of islet cells. We also determined the concentration levels of cytokines and insulin in the supernatant of the culture medium. We used anti-vascular endothelial growth factor (VEGF) and anti-interleukin (IL)-6 receptor antibodies to investigate the effect of VEGF and IL-6 on islet cells. RESULTS: ADSCs improved the viability of islet cells in the absence of cell-cell contact (P < 0.05). VEGF and IL-6 levels in the culture medium increased when islet cells were cocultured with ADSCs (P < 0.05). Furthermore, inhibition of VEGF decreased the viability of islet cells (P < 0.05); however, inhibition of IL-6 did not affect islet cell viability. CONCLUSIONS: These results suggested that trophic factors, particularly VEGF, secreted by human ADSCs enhanced the survival and function of porcine islet cells.
Authors: Simen W Schive; Mohammad Reza Mirlashari; Grete Hasvold; Mengyu Wang; Dag Josefsen; Hans Petter Gullestad; Olle Korsgren; Aksel Foss; Gunnar Kvalheim; Hanne Scholz Journal: Cell Med Date: 2017-04-14
Authors: Bianca Marmontel de Souza; Ana Paula Bouças; Fernanda Dos Santos de Oliveira; Karina Pires Reis; Patrícia Ziegelmann; Andrea Carla Bauer; Daisy Crispim Journal: Islets Date: 2017-03-04 Impact factor: 2.694
Authors: Gang Ren; Melika Rezaee; Mehdi Razavi; Ahmed Taysir; Jing Wang; Avnesh S Thakor Journal: Cell Tissue Res Date: 2019-02-01 Impact factor: 5.249
Authors: Ahmed A Arzouni; Andreia Vargas-Seymour; Nance Nardi; Aileen J F King; Peter M Jones Journal: Stem Cells Transl Med Date: 2018-05-11 Impact factor: 6.940
Authors: Ahmed A Arzouni; Andreia Vargas-Seymour; Paramjeet K Dhadda; Chloe L Rackham; Guo-Cai Huang; Pratik Choudhary; Aileen J F King; Peter M Jones Journal: Stem Cells Transl Med Date: 2019-05-08 Impact factor: 6.940