Literature DB >> 24234286

Rapid determination of anti-enzyme titer in serum using wholeStaphylococcus aureus cells.

R W McClard1.   

Abstract

Heat-killed, formalin-fixedStaphyloccous aureus (Cowan) cells are shown to be an excellent immunoadsorbent for quickly and accurately measuring the titer of antibodies directed against enzymes. The simple procedure is illustrated by following the course of immunization of a rabbit against mouse uridine 5't -monophosphate synthase. The antibody titer of a serum sample can be determined in roughly 1 h plus the time to perform the assays of enzyme (antigen) activity.

Entities:  

Year:  1982        PMID： 24234286     DOI： 10.1007/BF02798308

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  14 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

2.  Immunoprecipitation of specific polysomes using Staphylococcus aureus: purification of the immunoglobulin k chain messenger RNA from the mouse myeloma MPC11.

Authors:  N M Gough; J M Adams
Journal:  Biochemistry       Date:  1978-12-12       Impact factor: 3.162

3.  Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.

Authors:  E WASSERMAN; L LEVINE
Journal:  J Immunol       Date:  1961-09       Impact factor: 5.422

4.  Immunoassay of endogenous plasma insulin in man.

Authors:  R S YALOW; S A BERSON
Journal:  J Clin Invest       Date:  1960-07       Impact factor: 14.808

5.  A rapid sensitive assay for specific protein synthesis in cells and in cell-free translations: use of Staphylococcus aureus as an adsorbent for immune complexes.

Authors:  R D Ivarie; P P Jones
Journal:  Anal Biochem       Date:  1979-08       Impact factor: 3.365

6.  Use of immunoglobulin-loaded protein A-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay.

Authors:  E O'Keefe; V Vennett
Journal:  J Biol Chem       Date:  1980-01-25       Impact factor: 5.157

7.  Enzyme immunoassay ELISA and EMIT.

Authors:  E Engvall
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

8.  Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments.

Authors:  G Kronvall; D Frommel
Journal:  Immunochemistry       Date:  1970-01

9.  An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen.

Authors:  S Z Shapiro; J R Young
Journal:  J Biol Chem       Date:  1981-02-25       Impact factor: 5.157

10.  Isolation and initial characterization of the single polypeptide that synthesizes uridine 5'-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5'-monophosphate synthase.

Authors:  R W McClard; M J Black; L R Livingstone; M E Jones
Journal:  Biochemistry       Date:  1980-09-30       Impact factor: 3.162
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  1 in total

1.  Characterization of UMP synthase in dairy cattle heterozygous for a lethal recessive trait.

Authors:  K K Harden; J L Robinson
Journal:  Biochem Genet       Date:  1987-08       Impact factor: 1.890
  1 in total

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