Temperature-dependent behavior of immobilized alkaline phosphatase II. temperature pulse : A tool to enhance enzymatic activity for prolonged periods.

The specific activity of immobilized alkaline phosphatase can be considerably increased by exposure to a brief temperature pulse. The thus-pulsed preparations show a hysteresis of higher activity at room temperature varying from 10 min to several days, depending upon their pretreatment and the conditions of the reaction mixture during the pulse. In almost all cases the pulse can be repeated many times to increase the activity.