Literature DB >> 24233801

Immobilization ofmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD(+)) on the same matrix.

F Pittner1.   

Abstract

myo-Inositol-1-phosphate synthase (EC 5.5.1.4) from rat testes, an NAD(+)-containing enzyme, which convertsD-glucose 6-phosphate to 1L-myo-inositol 1-phosphate, could be immobilized together with its cofactor and bovine serum albumin by crosslinking with glutaraldehyde at pH 4.5. The enzyme bound to the gel showed a specific activity of 5.6% of that of the native enzyme, but the activity could be increased to 21% by pretreatment with urea.

Entities:  

Year:  1981        PMID： 24233801     DOI： 10.1007/BF02779242

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  9 in total

1.  Determination of nitrogen in proteins by means of idanetrione hydrate.

Authors:  S JACOBS
Journal:  Nature       Date:  1959-01-24       Impact factor: 49.962

2.  Chemical grafting of functional NAD in the active site of a dehydrogenase: regeneration in situ.

Authors:  M D Legoy; J M le Moullec; D Thomas
Journal:  FEBS Lett       Date:  1978-10-15       Impact factor: 4.124

3.  Preparation of homogeneous crystals of myo-inositol 1-phosphate synthase from rat testicles--further data on the chemical and catalytic properties of the enzyme (studies on the biosynthesis cyclitols, XXXIX).

Authors:  F Pittner; O Hoffmann-Ostenhof
Journal:  Mol Cell Biochem       Date:  1979-12-14       Impact factor: 3.396

4.  Studies on the biosynthesis of cyclitols, XXXVII. On mechanism and function of Schiff's base formation as an intermediary reaction step of myo-inositol-1-phosphate synthase from rat testicles.

Authors:  F Pittner; O Hoffmann-Ostenhof
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1978-10

5.  The application of immobilized NAD+ in an enzyme electrode and in model enzyme reactors.

Authors:  P Davies; K Mosbach
Journal:  Biochim Biophys Acta       Date:  1974-12-29

6.  Studies on the biosynthesis of cyclitols, XXX. Purification of myo-inositol-1-phosphate synthase of rat testes to homogeneity by affinity chromatography on NAD-sepharose.

Authors:  F Pittner; W Fried; O Hoffmann-Ostenhof
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1974-02

7.  The preparation of soluble high molecular weight NAD derivative active as a cofactor.

Authors:  J R Wykes; P Dunnill; M D Lilly
Journal:  Biochim Biophys Acta       Date:  1972-12-29

8.  Preparation of an alcohol-dehydrogenase--NAD(H)--sepharose complex showing no requirement of soluble coenzyme for its activity.

Authors:  S Gestrelius; M O Månsson; K Mosbach
Journal:  Eur J Biochem       Date:  1975-09-15

9.  A colorimetric determination of inositol monophosphates as an assay for D-glucose 6-phosphate-1L-myoinositol 1-phosphate cyclase.

Authors:  J E Barnett; R E Brice; D L Corina
Journal:  Biochem J       Date:  1970-09       Impact factor: 3.857
  9 in total
  1 in total

1.  Influence of urea and organic solvents on the activity of immobilizedmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD(+)) on the same matrix.

Authors:  F Pittner
Journal:  Appl Biochem Biotechnol       Date:  1981-06       Impact factor: 2.926
  1 in total

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