| Literature DB >> 24233789 |
Michelina Iacovino1, Megan E Roth, Michael Kyba.
Abstract
Embryonic stem cell (ESC) differentiation is a useful means by which to produce large quantities of cells in vitro representing early stages of embryonic development. A conditional gene expression system allows interrogation of factors at specific time points in the differentiation of ES cells to defined cell types. We have developed a method for rapidly generating conditional inducible murine ES cells by targeting genes into an Inducible Cassette Exchange (ICE) locus. The ICE locus encodes a doxycycline-inducible floxed Cre, which replaces itself with an incoming floxed gene of interest. The derivative cell lines, selected in G418, thus bear doxycycline-inducible transgenes. We provide detailed methods for performing ICE recombination and generating derivative doxycycline-inducible cell lines.Entities:
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Year: 2014 PMID： 24233789 PMCID： PMC3935508 DOI： 10.1007/978-1-62703-721-1_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745